Purified anti-Ubiquilin-2 Antibody

Pricing & Availability
Clone
A18118A (See other available formats)
Other Names
Chap1, DSK2 homolog, Protein linking IAP with cytoskeleton 2, PLIC-2, hPLIC-2, Ubiquitin-like product Chap1/Dsk2
Isotype
Mouse IgG2b, κ
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Product Citations
publications
A18118A_Pure_Ubiquilin-2_Antibody_1_062719
Western blot of purified anti-Ubiquilin-2 antibody (clone A18118A). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of rat brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A18118A_Pure_Ubiquilin-2_Antibody_1_062719
    Western blot of purified anti-Ubiquilin-2 antibody (clone A18118A). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of rat brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A18118A_Pure_Ubiquilin-2_Antibody_2_062719
    IHC staining of purified anti-Ubiquilin-2 antibody (clone A18118A) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • A18118A_Pure_Ubiquilin-2_Antibody_3_062719
    IHC staining of purified anti-Ubiquilin-2 antibody (clone A18118A) on formalin-fixed paraffin-embedded human glioblastoma tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
867901 25 µg £60
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867902 100 µg £158
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Description

Ubiqulin-2 is a ubiquitin-like protein that facilitates the degradation of ubiquitinated proteins by mediating their binding to the proteasome. Mutations in ubiquilin-2 have been linked to neurodegenerative diseases such as amyotrophic lateral sclerosis and frontal temporal dementia. These mutations are linked to disruption and impairment in the delivery of ubiquitinated substrates to the proteasome. In addition, ubiquilin-2 can interact with components of the ERAD machinery and localizes to autophagosomes, indicating that it plays a role in these pathways.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Ubqln2 recombinant protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 2.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
Human Ubiquilin-2 is a 624 amino acid protein with a predicted molecular mass of 65 kD and observed molecular mass of ~75 kD.
Distribution

Tissue distribution: Widely expressed, highest expression in cerebellar vermis
Cellular distribution: Nucleus, cytoplasm, membrane, and autophagosome

Function
Ubiquilin-2 plays an important role in the regulation of different protein degradation pathways including the ubiquitin-proteasome system, autophagy, and the endoplasmic reticulum-associated protein degradation.
Interaction
Ubiquilin-2 interacts with UBQLN1, UBE3A, BTRC, 19S proteasome subunit, C9orf72, HNRNPA1, and HNRNPU.
Cell Type
Neurons
Biology Area
Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Molecular Family
Autophagosome Markers
Antigen References
  1. Deng HX. et al. 2011. Nature. 477(7363):211-5.
  2. Hjerpe R. et al. 2016. Cell. 166(4):935-949. 
  3. Wu Q. et al. 2015. Acta Neuropathol. 129(3): 417–428.
Gene ID
29978 View all products for this Gene ID
UniProt
View information about Ubiquilin-2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/27/2019

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