- 10D8 (See other available formats)
- Regulatory Status
- Other Names
- α-tubulin, Tubulin, alpha-ubiquitous chain
- Mouse IgM, κ
- Ave. Rating
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- Product Citations
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Tubulin, one of the major constituents of microtubules, is a dimeric protein consisting of an alpha and beta chain. Tubulin is a highly conserved protein that is ubiquitously expressed. Tubulin is a GTP-binding protein that can be modified by phosphorylation and acetylation. Tubulin interacts with a variety of proteins including RAC GTPase activating protein 1, ZAP70, Polo-like kinase, c-Myc, Fyn, and microtubule associated protein 1A. The 10D8 antibody recognizes α-tubulin in all species and is useful for Western blotting, and immunofluorescence staining. The 10D8 antibody can also be used to monitor protein loading in Western blotting.Product Details
- Verified Reactivity
- Human, Mouse, Rat, All Species
- Antibody Type
- Host Species
- Recombinant full length human α-tubulin
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
WB - Quality tested
IHC-P - Verified
ICC - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5 µg per 5 ml antibody dilution buffer for each mini-gel. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 2 - 10 µg/mL is suggested. For immunofluorescent staining applications, a concentration range of 1 - 4 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
(PubMed link indicates BioLegend citation)
- Chou HYE, et al. 2006. J. Biol. Chem. 281:15201.
- Product Citations
AB_439760 (BioLegend Cat. No. 627901)
AB_439761 (BioLegend Cat. No. 627902)
- Tubulin family, tubulin is a dimeric protein consisting of an alpha and beta chain. Alpha tubulin has an approximate molecular weight of 50-55 kD.
- Tubulin is one of the major constituents of microtubules. Binds GTP, alpha chain contains a non-exchangeable GTP binding site
- RAC GTPase activating protein 1, ZAP70, Polo-like kinase, c-Myc, Fyn, Microtubule associated protein 1A
- Phosphorylation, acetylation
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Antigen References
1. Cowan NJ, et al. 1983. Mol. Cell. Biol. 3:1738.
2. Miller FD, et al. 1987. J. Cell Biol. 105:3065.
3. Wilde CD, et al. 1982. Proc. Natl. Acad. Sci. USA 79:96.
- Gene ID
- 84790 View all products for this Gene ID
- View information about Tubulin-alpha on UniProt.org
Related Pages & Pathways
Other FormatsView All Tubulin-α Reagents Request Custom Conjugation
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