Purified anti-TOP2A Antibody

Pricing & Availability
Clone
W18080B (See other available formats)
Other Names
DNA Topoisomerase II α, TOP2, DNA Gyrase
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W18080B_PURE_TOP2A_Antibody_1_070319.png
Whole cell extracts (15 µg protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-TOP2A Antibody, clone W18080B, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
  • W18080B_PURE_TOP2A_Antibody_1_070319.png
    Whole cell extracts (15 µg protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-TOP2A Antibody, clone W18080B, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
  • W18080B_PURE_TOP2A_Antibody_2_070319.png
    Whole cell extracts (15 µg protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-TOP2A Antibody, clone W18080B, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
  • W18080B_PURE_TOP2A_Antibody_3_070319.png
    HeLa cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with ice-cold methanol for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with a 1:100 dilution (5.0 µg/mL) of either Purified Rat IgG2a, κ isotype control antibody (Cat. No. 400602, panel A) or Purified anti-TOP2A Antibody (panel B) overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-rat IgG (Cat. No. 405422) at 2.0 µg/mL. Nuclei were counter-stained with DAPI, and the image was captured with a 60X objective.
Cat # Size Price Quantity Avail. Save
623701 25 µg £57
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623702 100 µg £144
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Description

DNA Topoisomerase-II α, or TOP2A, alters the toplogical states of DNA during various cellular processes, including transcription, meiosis, mitosis, and chromatin segregation. The enzyme functions by breaking and religating two strands of DNA, allowing each strand to pass through one another. Multiple classes of anti-cancer drugs initiate cell death by inceasing the frequency of potentially deleterious TOP2A-mediated DNA breaks.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant protein corresponding to amino acids 1232-1531 of TOP2A
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

W18080B failed IP testing.

When used for ICC, W18080B may produce light cytosolic staining. It is unclear if this represents background or TOP2A localized to the cytosol (Ramamoorthy M, et al. 2012. Nucleic Acids Res. 40:1621).

W18080B was ICC-tested on PFA-fixed HeLa cells permeabilized with Triton X-100 or methanol. The clone was compatible with both permeabilization methods.

Antigen Details

Structure
TOP2A is a 1,531 amino acid protein with a predicted molecular weight of 174 kD
Distribution

Nucleus/ubiquitously expressed

Function
Regulate DNA topology
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Chromatin Remodeling/Epigenetics, DNA Repair/Replication
Molecular Family
Enzymes and Regulators
Antigen References
  1. Champoux JJ. 1977. Proc. Natl. Acad. Sci. USA. 74:5328.
  2. Brown PO and Cozzarelli NR. 1979. Science. 206:1081.
Gene ID
7153 View all products for this Gene ID
UniProt
View information about TOP2A on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 07/03/2019

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