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- Clone
- O92A4 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Tollip, Toll Interacting Protein
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Western blot of purified anti-Tollip antibody (clone O9I2A4). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 854401 | 25 µg | £73 | ||||
| 854402 | 100 µg | £189 | ||||
Tollip, also known as Toll interacting protein, is a ubiquitin-binding protein and an inhibitory adaptor protein involved in IL-1 and Toll-like receptor (TLR) signaling pathway. Alterations in Tollip gene expression levels has been linked to tuberculosis and idiopathic pulmonary fibrosis, suggesting a role for Tollip in innate immune system. Tollip has also been reported to mediate autophagic clearance of poly-ubiquitinated proteins.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Full length human recombinant Tollip (NP_061882) produced in HEK293T cell.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This clone is highly specific to human Tollip. It does not cross react with murine Tollip.
- RRID
-
AB_2750411 (BioLegend Cat. No. 854401)
AB_2750472 (BioLegend Cat. No. 854402)
Antigen Details
- Structure
- Tollip is a 274 amino acid protein with an apparent molecular mass of ~30 kD.
- Distribution
-
Tissue Distribution: Ubiquitously expressed.
Cellular Distribution: Plasma membrane, extracellular, cytosol, lysosome, and nucleus. - Function
- Tollip is a component of the IL-1 and TLR signaling pathway involved in innate immune response.
- Interaction
- Tollip has been reported to interact with TOM1, TLR2, TLR4, IRAK1, IR-1RI, TOM1L2, and ILRAP.
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
- Molecular Family
- Enzymes and Regulators
- Antigen References
-
- Capelluto DG. 2012. Microbes Infect. 14(2): 140
- Brandao I, et al. 2015. Gut Microbes. 6(3):202
- Gene ID
- 54472 View all products for this Gene ID
- UniProt
- View information about Tollip on UniProt.org
Related FAQs
Other Formats
View All Tollip Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Tollip | O92A4 | WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Tollip
Western blot of purified anti-Tollip antibody (clone O9I2A4)...
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