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Purified anti-Thioredoxin 1 Antibody

Pricing & Availability
Clone
4H12A59 (See other available formats)
Regulatory Status
RUO
Other Names
Thioredoxin-1 (Trx1), Fip, FipA, TsnC, TrxA.
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
4H12A59_Thioredoxin_Purified_Antibody_WB_121213.jpg
Recombinant thioredoxin tagged TSC22D3 protein was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-Thioredoxin tag (clone 4H12A59) antibody. Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
  • 4H12A59_Thioredoxin_Purified_Antibody_WB_121213.jpg
    Recombinant thioredoxin tagged TSC22D3 protein was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-Thioredoxin tag (clone 4H12A59) antibody. Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
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658901 25 µg £70
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658902 100 µg £165
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Description

Thioredoxin is an 11.7 kD E. coli cytoplasmic protein that act as an anti-oxidant in the cells. When used as a fusion partner to produce recombinant protein, thioredoxin has been shown to increase protein solubility and yield, as well as reduce protein aggregation and precipitation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
E. coli
Reported Reactivity
Species independent
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length thioredoxin recombinant protein expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The 4H12A59 anti-thioredoxin antibody can be used for Western blotting to detect E. coli thioredoxin-1 and thioredoxin tagged recombinant protein. 

Product Citations
  1. Sinha D, et al. 2020. Anal Biochem. 113827:608. PubMed
RRID
AB_2783364 (BioLegend Cat. No. 658901)
AB_2562961 (BioLegend Cat. No. 658902)

Antigen Details

Structure
109 amino acids, predicted molecular weight of 12 kD.
Biology Area
Cell Biology
Antigen References

1. LaVallie ER, et al. 2003. Methods Mol. Biol. 205:119.
2. Lu Z, et al. 1996. J. Biol. Chem. 271:5059.
3. LaVallie ER, et al. 1993. Biotechnology. 11:187.

Gene ID
948289 View all products for this Gene ID
UniProt
View information about Thioredoxin-1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 09/19/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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