Purified anti-Thioredoxin 1 Antibody

Pricing & Availability
Clone
4H12A59 (See other available formats)
Other Names
Thioredoxin-1 (Trx1), Fip, FipA, TsnC, TrxA.
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
4H12A59_Thioredoxin_Purified_Antibody_WB_121213.jpg
Recombinant thioredoxin tagged TSC22D3 protein was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-Thioredoxin tag (clone 4H12A59) antibody. Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
  • 4H12A59_Thioredoxin_Purified_Antibody_WB_121213.jpg
    Recombinant thioredoxin tagged TSC22D3 protein was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-Thioredoxin tag (clone 4H12A59) antibody. Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
Cat # Size Price Quantity Avail. Save
658901 25 µg £57
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658902 100 µg £138
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Description

Thioredoxin is an 11.7 kD E. coli cytoplasmic protein that act as an anti-oxidant in the cells. When used as a fusion partner to produce recombinant protein, thioredoxin has been shown to increase protein solubility and yield, as well as reduce protein aggregation and precipitation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
E. coli Thioredoxin-1 and thioredoxin tagged recombinant protein.
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length thioredoxin recombinant protein expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The 4H12A59 anti-thioredoxin antibody can be used for Western blotting to detect E. coli thioredoxin-1 and thioredoxin tagged recombinant protein. 

RRID
AB_2783364 (BioLegend Cat. No. 658901)
AB_2562961 (BioLegend Cat. No. 658902)

Antigen Details

Structure
109 amino acids, predicted molecular weight of 12 kD.
Biology Area
Cell Biology
Antigen References

1. LaVallie ER, et al. 2003. Methods Mol. Biol. 205:119.
2. Lu Z, et al. 1996. J. Biol. Chem. 271:5059.
3. LaVallie ER, et al. 1993. Biotechnology. 11:187.

Gene ID
948289 View all products for this Gene ID
UniProt
View information about Thioredoxin-1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 12/12/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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