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Purified anti-TGM2 Antibody

Pricing & Availability
Clone
W17122A (See other available formats)
Regulatory Status
RUO
Other Names
C Polypeptide, Protein-Glutamine-Gamma-Glutamyltransferase, Protein-Glutamine Gamma-Glutamyltransferase 2, Transglutaminase C, Transglutaminase H, Tissue Transglutaminase, Transglutaminase 2
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
a-W17122A_PURE_TGM2_Antibody_051719
Total cell lysates (15 µg total protein) from MOLT-4 and HeLa cells (negative control), K562, A549, A431 and NIH/3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-TGM2 Antibody, clone W17122A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular Weight marker. Predicted TGM2 expression data was obtained from Human Protein Atlas.
  • a-W17122A_PURE_TGM2_Antibody_051719
    Total cell lysates (15 µg total protein) from MOLT-4 and HeLa cells (negative control), K562, A549, A431 and NIH/3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-TGM2 Antibody, clone W17122A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular Weight marker. Predicted TGM2 expression data was obtained from Human Protein Atlas.
  • b-W17122A_PURE_TGM2_Antibody_2_052223
    IHC staining of purified anti-TGM2 (clone W17122A) on formalin-fixed paraffin-embedded human placenta tissue. Following antigen retrieval using Tris-EDTA (10 mM Tris, 1 mM EDTA, 0.05% Tween-20, pH 9.0), the tissue was incubated without (panel A) and with (panel B) 1.0 µg/mL of purified anti-TGM2 (clone W17122A) overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Check Availability Save
616601 25 µg £70
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616602 100 µg £174
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Description

Transglutaminase 2 (TGM2) is a multifunctional enzyme required for diverse cellular processes, including cell death, differentiaton, apoptosis, and cell migration. TGM2 catalyzes the crosslinking through calcium-activated transamidation of available glutamine and lysine residues on substrate proteins. TGM2 also has GTPase and ATPase activity; transamidation activity is allosterically inhibited by guanine nucleotide binding. It is essential for cancer stem cell survival and is a chemotherapy resistance factor.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial human recombinant human TGM2 protein corresponding to amino acids 1-250.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone was tested for ICC using PFA-fixed A431 cells permeabilized with Triton X-100 or methanol. Staining in the incorrect subcellular compartment was observed using each method; we therefore do not recommend this clone for ICC.

This clone failed to immunoprecipitate TGM2 during PD testing.

RRID
AB_2810665 (BioLegend Cat. No. 616601)
AB_2810666 (BioLegend Cat. No. 616602)

Antigen Details

Structure
TGM2 is a 687 amino acid protein with a predicted molecular weight of 77 kD.
Distribution

Tissue enriched/cytosol localization

Function
Post translational modification, GTPase activity.
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Structure
Molecular Family
Enzymes and Regulators
Gene ID
7052 View all products for this Gene ID
UniProt
View information about TGM2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06/22/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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