Purified anti-TFIIB Antibody

Pricing & Availability
Clone
IIB1 (See other available formats)
Other Names
Transcription initiation factor IIB, General transcription factor TFIIB, S300-II, GTF2B, TF2B, TFIIB
Isotype
Mouse IgG2b
Ave. Rating
Submit a Review
Product Citations
publications
A-IIBI_Pureaf_TFIIB_Antibody_1_WB_090616
Total cell lysate from E. coli DH5α (lane 1, 15µg), NIH3T3 cells (lane 2, 15µg) and HeLa cells (lane 3, 15µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-TFIIB antibody (clone IIB1). Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection.
  • A-IIBI_Pureaf_TFIIB_Antibody_1_WB_090616
    Total cell lysate from E. coli DH5α (lane 1, 15µg), NIH3T3 cells (lane 2, 15µg) and HeLa cells (lane 3, 15µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-TFIIB antibody (clone IIB1). Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection.
  • B-IIBI_Pureaf_TFIIB_Antibody_2_WB_071018
    Total cell lysates (15 µg protein) from HeLa (lane 1) and NIH3T3 (lane 2) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1 µg/mL (1:1000) of Purified anti-TFIIB Antibody (clone IIB1, upper panel). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted anti-mouse-IgG secondary antibody conjugated to HRP for anti-TFIIB Antibody or 1:5000 diluted Direct-Blot™ HRP anti-β-Actin antibody, clone 2F1-1(lower panel) as a loading control. Lane M: Molecular weight ladder.
  • C-IIBI_Pureaf_TFIIB_Antibody_2_IF_050115
    HeLa cells were fixed with 2% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for five minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 2 µg/ml anti-TFIIB (clone IIB1) in blocking buffer overnight at 4°C and followed by DyLight™ 594 (red) conjugated goat anti-mouse IgG for one hour at room temperature. Actin filaments were labeled with Alexa Fluor® 488 Phalloidin (green). Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
Cat # Size Price Quantity Avail. Save
668501 100 µg £185
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

TFIIB is one of the ubiquitous transcription factors required for transcription initiation by RNA polymerase II. It plays an important role in pre-initiation complex assembly, which provides a bridge between promoter-bound TFIID and RNA polymerase II. Moreover, TFIIB also directly modulates the catalytic center of RNA polymerase II.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant protein.
Formulation
Phosphate-buffered solution, pH 7.2.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Validated
IP - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 µg per ml. For immunocytochemistry, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone IIB1 recognizes epitope 1-51.

Application References

(PubMed link indicates BioLegend citation)
  1. Thompson NE, et al. 1995. J. Biol. Chem. 270:4735. (IP)
RRID
AB_2564439 (BioLegend Cat. No. 668501)

Antigen Details

Structure
316 amino acids with a predicted molecular weight of approximately 35 kD.
Distribution

Nucleus.

Function
The Transcription factor is required for transcription initiation by RNA polymerase II.
Interaction
Transcription factors IID and IIA and RNA polymerase II.
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Thompson NE, et al. 1992. Biochemistry 31:7003.
2. Ha I, et al. 1991. Nature 352:689.
3. Hurwitz, J. 2005. J. Biol. Chem. 280:42477.
4. Deng W, Roberts SG. 2007. Chromosoma. 5:417.
5. Hirata A, et al. 2008. Nature 451:851.

Gene ID
2959 View all products for this Gene ID
UniProt
View information about TFIIB on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 07/10/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login / Register
Forgot your password? Reset password?
Create an Account