Purified anti-TCF1 (TCF7) Antibody

Pricing & Availability
Clone
W16175A (See other available formats)
Other Names
Transcription Factor 7, Transcription Factor 7 (T-Cell Specific, HMG-Box), T-Cell-Specific Transcription Factor 1, T-Cell Factor-7, T-Cell Factor 1, TCF1
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W16175A_PURE_TCF1_Antibody_1_073119.png
Whole cell extracts (15 µg total protein) NIH/3T3 (negative control), EL4, and MOLT-4 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-TCF1 (TCF7) Antibody, clone W16175A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular Weight marker.
  • W16175A_PURE_TCF1_Antibody_1_073119.png
    Whole cell extracts (15 µg total protein) NIH/3T3 (negative control), EL4, and MOLT-4 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-TCF1 (TCF7) Antibody, clone W16175A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular Weight marker.
  • W16175A_PURE_TCF1_Antibody_2_073119.png
    Whole cell extracts (300 µg total protein) prepared from Jurkat cells were immunoprecipitated overnight with 2.5 µg of Purified Rat IgG2a, κ Isotype Control Antibody (ISO, Cat. No. 400502) or Purified anti-TCF1 (TCF7) Antibody, clone W16175A (W16175A IP). The resulting IP fractions and whole cell extract input (5%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with a separate anti-TCF1/TCF7 antibody raised with a different immunizing sequence. Lane M: Molecular Weight marker.
Cat # Size Price Quantity Avail. Save
615701 25 µg £57
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615702 100 µg £144
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Description

TCF1 is the first identified member of the T-cell-specific transcription factor family.  It plays an important role in T cell development and differentiation. TCF1 is inactivated by association with the transcriptional repressor TLE proteins. During Wnt signaling, the transcriptional coactivator CTNNB1 accumulates and, in turn, replaces the transcriptional repressor associated with TCF1. Interaction with CTNNB1 results in transactivation of TCF1 target genes. Deletion of TCF1 causes massive apoptosis of double positive thymocytes, suggesting that TCF1 is required for thymocyte survival during T cell development. In addition to its function in the thymus, TCF1 promotes T cell differentiation to Th2 cells in the periphery through transcriptional activation of GATA3.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partical recombinant mouse TCF1 corresponding to amino acid residues 54-303
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. For immunoprecipitation, the suggested use of this reagent is 2.5 µg per IP. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

W16175A displayed a moderately higher affinity for TCF1/TCF7 than BioLegend clone 7F11A10 when tested for WB.

W16175A and 7F11A10 demonstrated similar performances when tested for IP.

W16175A stained HeLa cells, which lack TCF1/TCF7, when tested for ICC; we therefore do not recommend using this clone for the application.

W16175A recognizes both mouse and human TCF1/TCF7 by western blot; 7F11A10 is specific for human TCF1/TCF7.

Both W16175A and 7F11A10 recognize TCF1/TCF7 isoforms lacking the β-catenin binding domain.

Antigen Details

Structure
TCF1 is a 384 amino acid protein with a predicted molecular weight of 42 kD.
Distribution

Tregs/nuclear distribution

Function
TCF1 is a transcription factor, involved in the canonical Wingless/Integration 1 (Wnt) signaling pathway. TCF1 is essential for survival of CD4+/CD8+ double positive thymocytes and differentiation of T cells in the periphery.
Cell Type
T cells
Biology Area
Transcription Factors
Antigen References
  1. Germar K, et al. 2011. P.roc Natl. Acad. Sci. USA 108:20060-5.
  2. Wang R, et al. 2011. J. Immunol. 187:5964.
  3. Weber BN, et al. 2011. Nature 476:63.
  4. Jeannet G, et al. 2010. Proc. Natl. Acad. Sci. USA 107:9777-82.
  5. Staal FJ, et al. 1999. Int. Immunol. 11:317.

 

Gene ID
6932 View all products for this Gene ID
UniProt
View information about TCF1 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 07/31/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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