Purified anti-TBL1 Antibody

Pricing & Availability
Clone
4H2-D5-E9 (See other available formats)
Other Names
Transducin Beta Like 1 X-Linked, F-Box-Like/WD Repeat-Containing Protein TBL1X, SMAP55, EBI
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
4H2-D5-E9_PURE_TBL1_Antibody_1_100819
Western blot of purified anti-TBL1 antibody (4H2-D5-E9). Lane 1: Molecular weight marker; Lane 2: 10 µg of human HEK293 cell lysate; Lane 3: 10 µg of mouse NIH3T3 cell lysate; Lane 4: 20 µg of rat PC12 cell lysate. The blot was incubated with 1.0 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled Goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 4H2-D5-E9_PURE_TBL1_Antibody_1_100819
    Western blot of purified anti-TBL1 antibody (4H2-D5-E9). Lane 1: Molecular weight marker; Lane 2: 10 µg of human HEK293 cell lysate; Lane 3: 10 µg of mouse NIH3T3 cell lysate; Lane 4: 20 µg of rat PC12 cell lysate. The blot was incubated with 1.0 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled Goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 4H2-D5-E9_PURE_TBL1_Antibody_2_100819
    IHC staining of purified anti- TBL1 antibody (4H2-D5-E9) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 5.0 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 4H2-D5-E9_PURE_TBL1_Antibody_3_100819
    IHC staining of purified anti- TBL1 antibody (4H2-D5-E9) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 5.0 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
Cat # Size Price Quantity Avail. Save
869701 25 µg £60
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869702 100 µg £158
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Description

TBL1 is a protein that has sequence similarity with WD40 repeat-containing protein family. TBL1 plays an essential role in signal transduction, gene regulation, vesicle trafficking, and cytoskeletal arrangement through protein-protein interactions. TBL1 is also found as a component of nuclear receptor corepressor complexes through interaction with histone deacetylase 3, and is involved in Notch signaling. It is associated with diseases including ocular albinism and atrial septal defect 4.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified recombinant human TBL1 protein fragments expressed in E.coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 2.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
TBL1 is a 577 amino acid protein with a predicted and observed molecular mass of ~62 kD.
Distribution

Tissue Distribution: Brain, kidney, liver, heart, and lung
Cellular Distribution: Nucleus

Function
TBL1 is involved in the regulation of gene expression, RNA processing, vesicle trafficking, and cytoskeleton assembly.
Interaction
HDAC3, Notch1
Cell Type
Neurons
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Signal Transduction
Molecular Family
Cytoskeletal Proteins, Nuclear Markers
Antigen References
  1. Perissi V, et al. 2004. Cell. 116(4):511-26.
  2. Li J, et al. 2008. Nat Cell Biol. 10(2):160-9.
Gene ID
6907 View all products for this Gene ID 21372 View all products for this Gene ID 302711 View all products for this Gene ID
UniProt
View information about TBL1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/08/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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