Purified anti-Tau, x-421 Antibody (Previously Covance catalog# SIG-39410)

Pricing & Availability
Clone
C3 (See other available formats)
Other Names
Microtubule-associated protein tau, PHF-tau, paired helical filament-tau, neurofibrillary tangle, microtubule-associated protein tau, isoform 4, G protein beta1/gamma2 subunit-interacting factor 1, DDPAC, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND
Previously
Signet Catalog# 9410-02
Signet Catalog# 9410-05
Signet Catalog# 9410-10
Covance Catalog# SIG-39410
Isotype
Mouse IgG1
Ave. Rating
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Product Citations
publications
C3_PUREAF_Tau_x-421_IHC-P_050319
IHC staining of purified anti-Tau, x-421 antibody (clone C3) on formalin-fixed, paraffin-embedded diseased human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with the primary antibody at 10 µg/mL overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit was used for detection followed by hematoxylin counterstaining.
  • C3_PUREAF_Tau_x-421_IHC-P_050319
    IHC staining of purified anti-Tau, x-421 antibody (clone C3) on formalin-fixed, paraffin-embedded diseased human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with the primary antibody at 10 µg/mL overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit was used for detection followed by hematoxylin counterstaining.
Cat # Size Price Quantity Avail. Save
806304 50 µl £64
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806301 200 µl £233
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806302 500 µl £502
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Description

Tau proteins are microtubule-associated protein (MAPs) which are abundant in neurons of the central nervous system, but are also expressed at very low levels in CNS astrocytes and oligodendrocytes and elsewhere. One of tau's main functions is to modulate the stability of axonal microtubules. Tau is active primarily in the distal portions of axons providing microtubule stabilization as well as flexibility. Pathologies and dementias of the nervous system such as Alzheimer's disease feature tau proteins that have become defective and no longer stabilize microtubules properly. As a result, tau forms aggregates with specific structural properties referred to as Paired Helical Filaments (PHFs) that are a characteristic of many different types of dementias, known as tauopathies.

Tau has two primary ways of controlling microtubule stability: isoforms and phosphorylation. Six tau isoforms exist in human brain tissue, and they are distinguished by the number of binding domains. Three isoforms have three binding domains and the remaining three have four binding domains. The binding domains are located in the carboxy-terminus of the protein and are positively-charged (for binding to the negatively-charged microtubule). Tau isoforms with four binding domains are better at stabilizing microtubules than those with three binding domains.

Thus, in the human brain, the tau proteins constitute a family of six isoforms with the range from 352-441 amino acids. They also differ in either zero, one or two inserts of 29 amino acids at the N-terminal part (exon 2 and 3), and three or four repeat-binding regions at the C-terminus. So, the longest isoform in the CNS has four repeats (R1, R2, R3 and R4) and two inserts (441 amino acids total), while the shortest isoform has three repeats (R1, R3 and R4) and no insert (352 amino acids total). Tau is also a phosphoprotein with 79 potential Serine (Ser) and Threonine (Thr) phosphorylation sites on the longest tau isoform. Phosphorylation has been reported on approximately 30 of these sites in normal tau proteins. Mechanisms that drive tau lesion formation in the highly prevalent sporadic form of AD are not fully understood, but appear to involve abnormal post-translational modifications (PTMs) that influence tau function, stability, and aggregation propensity.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution (no preservatives or carrier proteins).
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

IHC-P - Quality tested
WB - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting, immunohistochemistry (IHC).

This antibody is specific for Tau cleaved at D421.

Application References

(PubMed link indicates BioLegend citation)
  1. Guillozet-Bongaarts AL, et al. 2005. Neurobiol. Aging. 26(7):1015-22. (WB, IHC)
  2. Horowitz PM, et al. 2004. J. Neurosci. 24(36):7895-902. (IHC)
  3. Arnaud LT, et al. 2009. J. Neurochem. 1:328. (WB, IF)
  4. Hamano T, et al. 2009. Int. J. Clin. Exp. Pathol. 6:561. (WB)
  5. Irwin DJ, et al. 2012. Brain 135:807. (IHC)
  6. Kilger E, et al. 2010. Proc. Natl. Acad. Sci. USA 17:7969. (IF, IHC)
RRID
AB_2715856 (BioLegend Cat. No. 806304)
AB_2564702 (BioLegend Cat. No. 806301)
AB_2564703 (BioLegend Cat. No. 806302)

Antigen Details

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
Tau
Gene ID
4137 View all products for this Gene ID
UniProt
View information about Tau, x-421 on UniProt.org

Related FAQs

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Go To Top Version: 5    Revision Date: 01/12/2017

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