- Poly28647 (See other available formats)
- Other Names
- Serine palmitoyltransferase 1, Long chain base biosynthesis protein 1, LCB 1, Serine-palmitoyl-CoA transferase 1, SPT1, Serine Palmitoyltransferase Long Chain Base Subunit 1
- Rabbit Polyclonal IgG
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Serine-palmitoyltransferase (SPT) catalyzes the rate-limiting step of the de novo synthesis of sphingolipids. SPT functions as heterodimers composed of two subunits, SPTLC1 (~ 55 kD), SPTLC2 (~65 kD) or SPTLC3 (~63 kD). SPT activity is detected in many tissues, including in the brain, lung, liver, kidney, and muscle. SPT activity is essential for embryonic development as SPTLC1 and SPTLC2 knockout mice die during embryogenesis. SPT may also be involved in the pathogenesis of sporadic Alzheimer’s disease (AD) due to its function in ceramide synthesis. In a subgroup of sporadic AD patients, the levels of ceramide and SPT are markedly elevated.Product Details
- Antibody Type
- Host Species
- Recombinant human SPTLC1 protein (Asp143-Leu473) expressed in E.coli.
- 0.01 M PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C
WB - Quality tested
IHC-P - Validated
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 2 - 5 µg per ml. For formalin-fixed paraffin-embedded immunohistochemical staining, a concentration range of 2 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Human SPTLC1 is a 473 amino acid protein with a predicted molecular mass of 53 kD.
Tissue distribution: Widely expressed
Cellular distribution: Endoplasmic reticulum
- SPTLC1 is involved in the de novo synthesis of sphingolipids.
- SPTLC1 interacts with SPTLC2 and SPTLC3.
- Cell Type
- Biology Area
- Cancer Biomarkers, Cell Biology, Neurodegeneration, Neuroinflammation, Neuroscience, Synaptic Biology
- Molecular Family
- Endoplasmic Reticulum Markers, Enzymes and Regulators, Organelle Markers
- Antigen References
- Hornemann T, et al. 2006. J Biol Chem. 281(49):37275-81.
- Geekiyanage H and Chan C. 2011. J Neurosci. 31(41): 14820–30.
- Gene ID
- 10558 View all products for this Gene ID
- View information about SPTLC1 on UniProt.org
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