Purified anti-SPTLC1 Antibody

Pricing & Availability
Clone
Poly28647 (See other available formats)
Other Names
Serine palmitoyltransferase 1, Long chain base biosynthesis protein 1, LCB 1, Serine-palmitoyl-CoA transferase 1, SPT1, Serine Palmitoyltransferase Long Chain Base Subunit 1
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
Poly28647_PURE_SPTLC1_Antibody_1_070319.png
Western blot of purified anti-SPTLC1 antibody (Poly28647). Lane 1: Molecular weight marker; Lane 2: 20 µg of human placenta lysate; Lane 3: 20 µg of HepG2 cell lysate. The blot was incubated with 2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled donkey anti-rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28647_PURE_SPTLC1_Antibody_1_070319.png
    Western blot of purified anti-SPTLC1 antibody (Poly28647). Lane 1: Molecular weight marker; Lane 2: 20 µg of human placenta lysate; Lane 3: 20 µg of HepG2 cell lysate. The blot was incubated with 2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled donkey anti-rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28647_PURE_SPTLC1_Antibody_2_070319.png
    IHC staining of purified anti-SPTLC1 antibody (Poly28647) on formalin-fixed paraffin-embedded human glioblastoma tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • Poly28647_PURE_SPTLC1_Antibody_3_070319.png
    IHC staining of purified anti-SPTLC1 antibody (Poly28647) on formalin-fixed paraffin-embedded Alzheimer’s disease brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • Poly28647_PURE_SPTLC1_Antibody_4_070319.png
    IHC staining of purified anti-SPTLC1 antibody (Poly28647) on formalin-fixed paraffin-embedded human placenta tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
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864701 25 µg £64
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Description

Serine-palmitoyltransferase (SPT) catalyzes the rate-limiting step of the de novo synthesis of sphingolipids. SPT functions as heterodimers composed of two subunits, SPTLC1 (~ 55 kD), SPTLC2 (~65 kD) or SPTLC3 (~63 kD). SPT activity is detected in many tissues, including in the brain, lung, liver, kidney, and muscle. SPT activity is essential for embryonic development as SPTLC1 and SPTLC2 knockout mice die during embryogenesis. SPT may also be involved in the pathogenesis of sporadic Alzheimer’s disease (AD) due to its function in ceramide synthesis. In a subgroup of sporadic AD patients, the levels of ceramide and SPT are markedly elevated.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Recombinant human SPTLC1 protein (Asp143-Leu473) expressed in E.coli.
Formulation
0.01 M PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 2 - 5 µg per ml. For formalin-fixed paraffin-embedded immunohistochemical staining, a concentration range of 2 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
Human SPTLC1 is a 473 amino acid protein with a predicted molecular mass of 53 kD.
Distribution

Tissue distribution: Widely expressed
Cellular distribution: Endoplasmic reticulum

Function
SPTLC1 is involved in the de novo synthesis of sphingolipids.
Interaction
SPTLC1 interacts with SPTLC2 and SPTLC3.
Cell Type
Neurons
Biology Area
Cancer Biomarkers, Cell Biology, Neurodegeneration, Neuroinflammation, Neuroscience, Synaptic Biology
Molecular Family
Endoplasmic Reticulum Markers, Enzymes and Regulators, Organelle Markers
Antigen References
  1. Hornemann T, et al. 2006. J Biol Chem.  281(49):37275-81.
  2. Geekiyanage H and Chan C. 2011. J Neurosci. 31(41): 14820–30.
Gene ID
10558 View all products for this Gene ID
UniProt
View information about SPTLC1 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 07/03/2019

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