IHC staining of purified anti-SOX-10 antibody (clone BSB-62) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-Mouse IgG (Cat. No. 405326) for one hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm.
IHC staining of purified anti-SOX-10 antibody (clone BSB-62) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-Mouse IgG (Cat. No. 405326) for one hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm.
IHC staining of purified anti-SOX-10 antibody (clone BSB-62) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-Mouse IgG (Cat. No. 405326) for one hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm.
IHC staining of purified anti-SOX-10 antibody (clone BSB-62) on formalin-fixed paraffin-embedded rat and mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The images were captured with a 40X objective. Scale bar: 50 µm
IHC staining of anti-SOX-10 antibody (clone BSB-62) on formalin-fixed paraffin-embedded human glial tumor tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/mL of the primary antibody for 30 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The images were captured with a 40X objective.
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 μg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes
This antibody may show weaker reactivity to human tissues.
Product Citations
Dravis C et al. 2018. Cancer cell. 34(3):466-482 . PubMed
SOX-10 is a 466 amino acid protein with a molecular mass of 50 kD.
Distribution
Tissue distribution: primarily expressed in the nuclei of glia in fetal and adult brain, and in the heart, salivary gland and skin.
Cellular distribution: nucleus.
Function
SOX-10 likely functions synergistically with the POU domain protein TST-1/OCT6/SCIP to confer function of other transcription factors in developing and mature glia. The protein acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development.
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