Purified anti-SLP-76 Antibody

Pricing & Availability
Clone
H76 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte cytoplasmic protein 2 (LCP2), SH2 domain containing leukocyte 76kD protein
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
1_H76_Purified_SLP-76_Antibody_1_052219.png
Total cell lysates (15 µg total protein) from HeLa (negative control), Daudi (negative control), THP-1, and HEL cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.5 µg/mL (1:1000 dilution) of purified anti-SLP-76 antibody, clone H76, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405301) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 664804) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker. Predicted expression data was obtained from Human Protein Atlas.
  • 1_H76_Purified_SLP-76_Antibody_1_052219.png
    Total cell lysates (15 µg total protein) from HeLa (negative control), Daudi (negative control), THP-1, and HEL cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.5 µg/mL (1:1000 dilution) of purified anti-SLP-76 antibody, clone H76, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405301) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 664804) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker. Predicted expression data was obtained from Human Protein Atlas.
  • 2_H76_Purified_SLP-76_Antibody_2_052219.png
    Whole cell extracts (15 µg protein) from serum starved Jurkat and EL4 cells untreated (-) or treated (+) with 5 mM H2O2 were resolved on a 4-12% Bis-Tris gel, transferred to nitrocellulose and probed with 0.1 µg/mL (1:5000 dilution) of purified anti-SLP76 Phospho (Ser376), clone A18001A (Cat. No. 667102), for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405301) at a 1:3000 dilution. Equal SLP76 loading was confirmed by probing membranes with anti-pan SLP76 antibody, clone H76 (Cat. No. 625002) at a 1:1000 dilution. Lane M: Molecular Weight marker.
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625002 200 µl (20 Western blots) £201
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Description

SLP-76 is a human Src homology domain-containing leukocyte protein. This cytoplasmic adaptor phosphoprotein contains both SH2 and SAM domains and is involved in B and T cell receptor signaling. The amino terminus of this protein contains three 17 amino acid repeats with conserved tyrosine and acidic residues (DYE(S/P)P) as well as a proline rich region and is known to associate with many proteins involved in T and B cell signaling including Grb2, LAT, Vav1, SLAP-130, SHP-1, and phospholipase C gamma 2. SLP-76 can be phosphorylated on multiple tyrosine residues by the upstream kinases ZAP-70 and Lck. SLP-76 phosphorylation plays an important role in T cell-mediated IL-2 production by allowing phosphorylated Vav to bind; this complex (SLP-76/Vav) stimulates NF-AT and IL-2 gene activation after TCR engagement. Overexpression of SLP-76 has been shown to result in enhanced IL-2 transcription after TCR signaling. The H76 monoclonal antibody recognizes human SLP-76 and has been shown to be useful for Western blotting.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
amino acids 216-434 of human SLP-76
Formulation
This antibody is provided in phosphate-buffered solution, containing 0.1% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
Upon receipt, aliquot and store frozen at -20°C. Avoid freeze-thaw cycles.
Application

WB - Quality tested
IP, FC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 1:1000 dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Deford-Watts LM, et al. 2011. J. Immunol. 186:6839. PubMed.
Product Citations
  1. Bacchelli C, et al. 2017. J Allergy Clin Immunol. 139:634. PubMed
  2. DeFord-Watts L, et al. 2011. J Immunol. 186:6839. PubMed
RRID
AB_2136888 (BioLegend Cat. No. 625002)

Antigen Details

Structure
Phosphoprotein 76 kD containing SH2 domain in the C-terminus
Distribution

Expressed in leukocytes (monocytes, T and B cells), thymus, spleen, placenta, and lung.

Function
Adaptor molecule involved in B cell receptor and T cell receptor signaling
Interaction
Grb2, LAT, Vav1, SLAP-130, SHP-1, phospholipase C gamma 2
Modification
Phosphorylation (Y113, Y128, Y145, Y423, Y426)
Cell Type
B cells, Monocytes, T cells
Biology Area
Cell Biology, Immunology, Signal Transduction
Molecular Family
Protein Kinases/Phosphatase
Antigen References

1. Jackman KJ, et al. 1995. J. Biol. Chem. 270:7029.
2. Wardenberg JB, et al. 1996. J. Biol. Chem. 271:19641.
3. Tuosto L, et al. 1996. J. Exp. Med. 184:1161.
4. Motto DG, et al. 1996. J. Exp. Med. 183:1937.

Gene ID
3937 View all products for this Gene ID
UniProt
View information about SLP-76 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 05/22/2019

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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