Purified anti-SLC6A1 Antibody

Pricing & Availability
Clone
A18095C (See other available formats)
Other Names
GAT-1, GABA Transporter
Isotype
Mouse IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
A18095C_PURE_SLC6A1_Antibody_062419
Western blot of purified anti-SLC6A1 antibody (clone A18095C). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A18095C_PURE_SLC6A1_Antibody_062419
    Western blot of purified anti-SLC6A1 antibody (clone A18095C). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Avail. Save
867401 25 µg £60
Check Availability


Need larger quantities of this item?
Request Bulk Quote
867402 100 µg £158
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

SLC6A1 is the gene that encodes GABA transporter 1 (GAT1) and functions as the major presynaptic protein in neurons involved in the removal of excessive GABA from the synaptic cleft and the extrasynaptic space. Diseases associated with GAT1 include neurodevelopmental disorders such as schizophrenia and seizure.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KLH-conjugated C-terminal peptide of mouse SLC6A1.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 2.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Avoid boiling lysates before loading to SDS for WB.

Antigen Details

Structure
GAT1 is a 599 amino acid protein with predicted and observed molecular mass of ~ 67 kD.
Distribution

Tissue Distribution: Brain
Cellular Distribution: Plasma membrane

Function
GAT1 is a GABA transporter protein that removes excessive GABA from the synaptic cleft and the extrasynaptic space.
Interaction
MPP5, STX1A
Ligand/Receptor
GABA
Cell Type
Astrocytes, GABAergic Neurons, Mature Neurons, Neurons, Oligodendrocytes
Biology Area
Neurodegeneration, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
Molecular Family
Neurotransmitter Receptor Trafficking/Endocytosis, Presynaptic proteins, Synaptic Vesicle Trafficking/Endocytosis
Antigen References
  1. Zafar S, et al. 2018. Front Chem. 6:397.
  2. Lie MEK, et al. 2017. Adv. Neurobiol. 16:137-167.
  3. Zhou Y, Danbolt NC. 2013. Front Endocrinol (Lausanne). 4:165.
Gene ID
6529 View all products for this Gene ID 232333 View all products for this Gene ID 79212 View all products for this Gene ID
UniProt
View information about SLC6A1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/24/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login / Register
Forgot your password? Reset password?
Create an Account