Purified anti-SH2B3 (LNK) Antibody

Pricing & Availability
Clone
O92B11 (See other available formats)
Other Names
SH2B Adaptor Protein 3, Lymphocyte-Specific Adapter Protein Lnk, Signal Transduction Protein Lnk, IDDM20, LNK, Lymphocyte Adapter Protein
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
O92B11_PURE_SH2B3_Antibody_1_082319
Total cell lysates (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-SH2B3/LNK Antibody, clone O92B11, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • O92B11_PURE_SH2B3_Antibody_1_082319
    Total cell lysates (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-SH2B3/LNK Antibody, clone O92B11, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
Cat # Size Price Quantity Avail. Save
671101 25 µg £57
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671102 100 µg £144
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Description

SH2B3, originally described as LNK, is a member of the SH2B adaptor family of proteins that controls signaling activities from activated growth factor and cytokine receptors. This protein functions as a negative regulator of cytokine signaling and plays a critical role in hematopoiesis and proliferation. Through an SH2 domain, SH2B3 has been shown to directly bind and inhibit downstream cytokine effectors, including Janus Kinases and other tyrosine kinases. SH2B3 functions as potent tumor suppressor in myeloproliferative disorders, while elevated SH2B3 has been described to confer a pro-survival advantage against the pro-apoptotic effects of interferon in cutaneous melanomas.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full-length recombinant human SH2B3 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

O92B11 was not tested for ICC or IP

RRID
AB_2814528 (BioLegend Cat. No. 671101)
AB_2814529 (BioLegend Cat. No. 671102)

Antigen Details

Structure
SH2B3/LNK is a 575 amino acid protein with a predicted molecular weight of 63 kDa
Distribution

Cytoplasm, plasma membrane and nucleus/highly-expressed in hematopoietic cells

Function
Negative regulator of JAK/STAT pathway
Cell Type
Hematopoietic stem and progenitors
Biology Area
Signal Transduction
Antigen References
  1. Oh ST, et al. 2010. Blood. 116:988.
  2. Perez-Garcia A, et al. 2013. Blood. 122:2425
  3. Lee S, et al. 2015. Oncotarget. 6:17764
  4. Ding LW, et al. 2019. Nat. Commun. 10:2230.
Gene ID
10019 View all products for this Gene ID
UniProt
View information about SH2B3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08/23/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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