Purified anti-RPS6 Phospho (Ser244) Antibody

Pricing & Availability
Clone
A18024B (See other available formats)
Other Names
Ribosomal Protein S6, 40S Ribosomal Protein S6, Phosphoprotein NP33, Small Ribosomal Subunit Protein ES6
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A18024B_PURE_RPS6-Phospho_Antibody_1_093019
Total cell lysates (15 µg protein) from serum-starved SR cells treated without (-) or with (+) 160 nM PMA for 30 minutes were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.1 µg/mL (1:5000 dilution) of Purified anti-RPS6 Phospho (Ser244) Antibody (clone A18024B). Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Equal protein loading was confirmed using a pan RPS6 antibody and Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) used at a 1:25000 dilution (lower). Lane M: Molecular Weight ladder.
  • A18024B_PURE_RPS6-Phospho_Antibody_1_093019
    Total cell lysates (15 µg protein) from serum-starved SR cells treated without (-) or with (+) 160 nM PMA for 30 minutes were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.1 µg/mL (1:5000 dilution) of Purified anti-RPS6 Phospho (Ser244) Antibody (clone A18024B). Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Equal protein loading was confirmed using a pan RPS6 antibody and Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) used at a 1:25000 dilution (lower). Lane M: Molecular Weight ladder.
  • A18024B_PURE_RPS6-Phospho_Antibody_2_093019
    Total cell lysates (15 µg protein) from serum-starved NIH/3T3 cells treated without (-) or with (+) 20% FBS for 30 minutes were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.1 µg/mL (1:5000 dilution) of Purified anti-RPS6 Phospho (Ser244) Antibody (clone A18024B). Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Equal protein loading was confirmed using a pan RPS6 antibody and Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) used at a 1:25000 dilution (lower). Lane M: Molecular Weight ladder.
  • A18024B_PURE_RPS6-Phospho_Antibody_3_093019
    Serum starved NIH/3T3 cells were untreated (panel A) or treated (panel B) with 20% FBS for 30 minutes. Cells were then fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with 5 µg/mL (1:100 dilution) of Purified anti-RPS6 Phospho (Ser244) Antibody overnight at 4°C, after which proteins were visualized with Alexa Fluor® 594 Goat anti-mouse IgG Antibody (Cat. No. 405326) at 2.0 µg/mL. Nuclei were counterstained with DAPI, and the image was captured with a 60X objective.
Cat # Size Price Quantity Avail. Save
933701 25 µg £64
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933702 100 µg £151
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Description

Ribosomal protein S6 (RPS6) is a key component of the small 40S ribosomal subunit and is the major substrate of protein kinases in eukaryotic ribosomes. In response to various cellular stimuli such as mitogenic stimulation, insulin, and increased nutrient availability, upstream kinases such as RSK and p70 kinases phosphorylate RPS6 at multiple serine sites. These modifications facilitate the recruitment of the 7-methylguanasine cap complex, thereby promoting the assembly of the translational pre-initiation complex and increased cellular protein synthesis capacity. RPS6 has been shown to be hyperphosphorylated in certain cancers, and phosphorylation is a critical determinant of pancreatic β–cell size and systemic glucose homeostasis function in diabetic mouse models.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide corresponding to human S6 phosphorylated at serine 244
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Validated

Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone is predicted to react with rat S6 when phosphorylated at serine 244 due to complete sequence homology between the immunizing sequence and the rat S6 ortholog.

Antigen Details

Structure
RPS6 is a 249 amino acid protein with a predicted molecular weight of 28.6 kD.
Distribution

Cytosol, nucleus, ER/ubiquitous expression

Function
Regulation of protein translation
Biology Area
Cell Biology, Cell Proliferation and Viability, Protein Synthesis, Signal Transduction
Molecular Family
Organelle Markers, Phospho-Proteins
Antigen References
  1. Jefferies HB, et al. 1997. EMBO J. 16:3693.
  2. Ruvinsky I, et al. 2005. Genes. Dev. 18
  3. Schumacher AM, et al. 2006. Biochemistry. 45:13614
  4. Roux PP, et al. 2007. J. Biol. Chem. 282:14056.
  5. Stevens C, et al. 2009. J. Biol. Chem. 284:334.
  6. Schlafli P, et al. 2011. FEBS J. 278:1757.
Gene ID
6194 View all products for this Gene ID
UniProt
View information about RPS6 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09/30/2019

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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