Purified anti-RORγ Antibody

Pricing & Availability
Clone
RORg2 (See other available formats)
Regulatory Status
RUO
Other Names
RORc, Nuclear receptor ROR-gamma, Nuclear receptor RZR-gamma
Isotype
Armenian hamster IgG
Ave. Rating
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Product Citations
publications
RORg2_PURE_RORy_Antibody_WB_082018
Total cell lysates (15 µg protein) from mouse thymus (lane 1, 2), mouse spleen (lane 3) and Molt-4 cells (lane 4) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 0.5 µg/mL (1:1000 dilution, lane 1) or 2 µg/mL (1:250 dilution, lane 2, 3, 4) of purified anti-RORγ antibody, clone RORg2 (upper panel). Proteins were visualized by chemiluminescence detection (Cat. No. 426303) using 1:3000 diluted anti-hamster-IgG secondary antibody conjugated to HRP for purified anti-RORγ antibody or 1:10000 diluted Direct-Blot™ HRP anti-β-actin antibody, clone W16197A (lower panel). Lane M: Molecular weight ladder.
  • RORg2_PURE_RORy_Antibody_WB_082018
    Total cell lysates (15 µg protein) from mouse thymus (lane 1, 2), mouse spleen (lane 3) and Molt-4 cells (lane 4) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 0.5 µg/mL (1:1000 dilution, lane 1) or 2 µg/mL (1:250 dilution, lane 2, 3, 4) of purified anti-RORγ antibody, clone RORg2 (upper panel). Proteins were visualized by chemiluminescence detection (Cat. No. 426303) using 1:3000 diluted anti-hamster-IgG secondary antibody conjugated to HRP for purified anti-RORγ antibody or 1:10000 diluted Direct-Blot™ HRP anti-β-actin antibody, clone W16197A (lower panel). Lane M: Molecular weight ladder.
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646501 25 µg £86.00
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646502 100 µg £188.00
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Description

RORγ (Retinoid-related orphan receptor gamma) belongs to nuclear hormone receptor family, NR1 subfamily. RORγ has two isoforms: RORγ1 and RORγ2 (also known as RORγt). RORγ1 contains 516 amino acids and RORγt contains 495 amino acids (RORγt lacks the amino terminus). RORγ1 is expressed in thymus, kidney, liver, muscle and brown fat tissue but not white fat tissue. RORγt is specifically expressed in CD4+/CD8+ thymocytes and lymphoid tissue inducers (LTi). RORγ plays critical roles during thymopoiesis, T cell homeostasis, lymph nodes and Peyers patch development. Among two isoforms, RORγt is well studied and it promotes thymocyte differentiation into pro-inflammatory Th17 cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse RORγ
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Sun Z, et al. 2000. Science 288:2369
Product Citations
  1. Onder L et al. 2017. Immunity. 47(1):80-92 . PubMed
RRID
AB_2180302 (BioLegend Cat. No. 646501)
AB_2238503 (BioLegend Cat. No. 646502)

Antigen Details

Structure
RORγ has two isoforms: RORγ1 and RORγ2 (also known as RORγt). RORγ1 contains 516 amino acids and RORγt contains 495 amino acids (RORγt lacks the amino terminus).
Distribution

RORγ1 is expressed in thymus, kidney, liver, muscle and brown fat tissue but not white fat tissue. RORγt is specifically expressed in CD4+/CD8+ double positive thymocytes, Th17 cells and lymphoid tissue inducers (LTi).

Function
RORγ plays critical roles during thymopoiesis, T cell homeostasis, lymph nodes and Peyers patch development. Among two isoforms, RORγt is well studied and it promotes thymocyte differentiation into pro-inflammatory Th17 cells.
Cell Type
Thymocytes
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Medvedev A, et al. 1997. Genomics 46:93.
2. He YW, et al. 1998. Immunity 9:797.
3. Eberl G, et al. 2004. Nat. Immunol. 5:64.

Gene ID
19885 View all products for this Gene ID
UniProt
View information about ROR gamma on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 08/20/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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