Purified anti-Rb Antibody

Pricing & Availability
Clone
W18116A (See other available formats)
Other Names
RB Transcriptional Corepressor 1, Retinoblastoma-Associated Protein, Protein Phosphate 1, Regulatory Subunit 130
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W18116A_PURE_Rb_Antibody_1_093019.png
Whole cell lysates (15 µg) from the listed cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with a 1.0 µg/mL (1:500 dilution) of Purified anti-Rb antibody (clone W18116A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • W18116A_PURE_Rb_Antibody_1_093019.png
    Whole cell lysates (15 µg) from the listed cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with a 1.0 µg/mL (1:500 dilution) of Purified anti-Rb antibody (clone W18116A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • W18116A_PURE_Rb_Antibody_2_093019.png
    Whole cell lysates (250 µg) were prepared from MOLT-4 cells and then immunoprecipitated overnight with 2.5 µg of Purified Rat IgG2a, κ Isotype Ctrl Antibody (Cat. No. 400502) or Purified Rb Antibody (clone W18116A). The resulting IP fractions and 5% whole cell lysate input were resolved by 4-12% Bis-Tris gel electrophoresis and probed with anti-Rb Antibody (clone W18116A) followed by HRP goat anti-rat IgG, light chain specific antibody for detection.
  • W18116A_PURE_Rb_Antibody_3_093019.png
    HeLa cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with methanol for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with a 1:500 dilution (1.0 µg/mL) of either Purified Rat IgG2a, κ Isotype Ctrl Antibody (Cat. No. 400502) (Panel A) or Purified anti-Rb1 Antibody (clone W18116A) (Panel B) overnight at 4°C, followed by incubation with Alexa Fluor® 594 Goat anti-mouse IgG Antibody (Cat. No. 405326) at 2.0 µg/mL. Nuclei were counterstained with DAPI, and the image was captured with a 60X objective.
Cat # Size Price Quantity Avail. Save
933401 25 µg £57
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933402 100 µg £144
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Description

Rb is a key regulator of the cell cycle, more specifically during the transition from the G1 to S phases. The underphosphorylated, active form of Rb interacts directly with E2F1, leading to cell cycle arrest, while the hyperphosphorylated form decouples from E2F1, thus promoting the transcription of genes promoting entry into the S phase. Rb is directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human Rb protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP, ICC - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/mL. For immunocytochemistry, a concentration of 5 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
Rb is a 928 amino-acid protein with a predicted molecular weight of 110 kD.
Distribution

Localized to the nucleoplasm/Ubiquitously expressed.

Function
Regulator; tumor suppressor; chromatin stability
Interaction
E2F1 Transcription Factor
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Cycle/DNA Replication
Molecular Family
Tumor Suppressors
Antigen References
  1. Dunham A, et al. 2004.  Nature. 428(6982):522-8.
  2. Hogg A, et al. 1992. Oncogene. (7):1445-51.
  3. Cho HS, et al. 2012. Neoplasia. 14(6):476-86.
  4. Mori T, et al. 2011. Cell Cycle. 10(19):3284-99.
  5. Rose JE, et al. 2010.  Mol Med. 16(7-8):247-53.
Gene ID
5925 View all products for this Gene ID
UniProt
View information about Rb on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09/30/2019

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