Purified anti-rat CD80 Antibody

Pricing & Availability
Clone
3H5 (See other available formats)
Regulatory Status
RUO
Other Names
B7-1, B7, BB1, Ly-53
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
3H5_PE_090611
LPS-stimulated LOU rat splenocytes (3 days) were stained with CD80 (clone 3H5) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
  • 3H5_PE_090611
    LPS-stimulated LOU rat splenocytes (3 days) were stained with CD80 (clone 3H5) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
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200202 500 µg £150.00
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Description

CD80 is a 60 kD member of the immunoglobulin superfamily also known as B7-1, B7, and BB1. It is expressed on antigen presenting cells and activated T and B cells, and is a ligand for CD28 and CD152 (CTLA-4). CD80/CD28 interactions are important for T and B cell co-stimulation, while CD80/CD152 lead to the inhibition of T cell responses. The 3H5 antibody can block the co-stimulatory activity of CD80.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
HTLV-1-transformed Lewis-S1 rat T cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F, IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1-3, blocking of ligand binding1-3, and immunohistochemical staining of acetone-fixed frozen sections3. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 200208).

Application References

(PubMed link indicates BioLegend citation)
  1. Maeda, K., et al. 1997. Int. Immunol. 9:993. (IP, Block)
  2. Bluestone, J.A., 1995. Immunity 2:555. (IP, Block)
  3. Damoiseaux, J.G., et al. 1998. J. Leuk. Biol. 64:803. (IP, Block, IHC)
  4. Ho, CH., et al. 2007. J. Virol. 81:4837. PubMed
  5. Ho, C., et al. 2009. Carcinogenesis 30:1443. PubMed
Product Citations
  1. Ho C, et al. 2007. J Virol. 81:4837. PubMed
  2. Ho C, et al. 2009. Carcinogenesis. 30:1443. PubMed
RRID
AB_313843 (BioLegend Cat. No. 200202)

Antigen Details

Structure
Ig superfamily, 60 kD
Distribution

Antigen presenting cells, activated B cells and T cells

Function
T and B cell costimulation
Ligand/Receptor
CD28, CD152 (CTLA-4)
Cell Type
Antigen-presenting cells, B cells, T cells
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Costimulatory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Barclay, A.N. et al. 1997. The Leukocyte Antigen FactsBook. Academic Press.
2. Linsley, P.S., et al. 1991. J. Exp. Med. 174:561.
3. Salomon, B., et al. 2001. Ann. Rev. Immunol. 19:225.

Gene ID
25408 View all products for this Gene ID
UniProt
View information about CD80 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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