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Purified anti-rat CD62P (P-selectin) Antibody

Pricing & Availability
Clone
RP-2 (See other available formats)
Regulatory Status
RUO
Other Names
GMP-140, platelet activation-dependent granule membrane protein (PADGEM), GMP140
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
RP-2_Purified_CD62P_Antibody_FC_092915
Thrombin stimulated rat platelets were stained with purified CD62P (clone RP-2) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE.
  • RP-2_Purified_CD62P_Antibody_FC_092915
    Thrombin stimulated rat platelets were stained with purified CD62P (clone RP-2) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE.
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206002 100 µg £121
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Description

CD62P is a 140 kD type I transmembrane glycoprotein that is also known as P-selectin, platelet activation-dependent granule membrane protein (PADGEM), and GMP-140. It is expressed on activated platelets, megakaryocytes, and endothelial cells. CD62P is primarily stored in secretory α-granules in platelets and Weibel-Palade bodies in endothelial cells, and is rapidly relocated to the plasma membrane upon activation. The ligands for CD62P are CD162 and CD24. CD62P's primary functions are cell adhesion during neutrophil rolling, and platelet-neutrophil and platelet-monocyte interactions.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Thrombin activated rat platelets
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC, IHC-F, ELISA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats of this clone) include: immunofluorescence staining2, immunohistochemistry of acetone-fixed frozen sections2, and ELISA capture3.

Application References

(PubMed link indicates BioLegend citation)
  1. Walter UM, et al. 1997. Immunology. 92:290. (Blocking function)
  2. Morise Z, et al. 1998. Am. J. Physiol. 274:G246. (IF, IHC - frozen)
  3. Walter UM, et al. 1997. Hybridoma. 16:249. (ELISA capture)
Product Citations
  1. Zhao W, et al. 2021. J Thromb Haemost. 19:3113. PubMed
RRID
AB_2564112 (BioLegend Cat. No. 206002)

Antigen Details

Structure
Type I transmembrane glycoprotein, 140 kD
Distribution

Activated platelets, megakaryocytes, and endothelial cells.

Function
Adhesion, neutrophil rolling, platelet-neutrophil, and platelet-monocyte interactions.
Ligand/Receptor
CD162 (PSGL-1), CD24, and sialylated Lewis X
Cell Type
Endothelial cells, Megakaryocytes, Platelets
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. McEver R, et al. 1995. J. Biol. Chem. 270:11025.
2. Varki A. 1994. Proc. Natl. Acad. Sci. USA 91:7390.

Gene ID
25651 View all products for this Gene ID
UniProt
View information about CD62P on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/14/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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