Purified anti-Rac1 Antibody

Pricing & Availability
Clone
CPTC-RAC1-2 (See other available formats)
Other Names
Ras-related C3 botulinum toxin substrate 1, p21-RAC1
Isotype
Mouse IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
CPTC-RAC1-2_PURE_Rac1_Antibody_1_070919
IHC staining of purified anti-Rac1 antibody (clone CPTC-RAC1-2) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • CPTC-RAC1-2_PURE_Rac1_Antibody_1_070919
    IHC staining of purified anti-Rac1 antibody (clone CPTC-RAC1-2) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • CPTC-RAC1-2_PURE_Rac1_Antibody_2_070919
    IHC staining of purified anti-Rac1 antibody (clone CPTC-RAC1-2) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
Cat # Size Price Quantity Avail. Save
854101 25 µg £60
Check Availability


Need larger quantities of this item?
Request Bulk Quote
854102 100 µg £158
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Rac1, Rac2 and Rac3 are three isoforms of Rac protein that exist in mammals. Rac proteins belong to the Rho-GTPase family, which regulate many cellular processes. Rac1 has been reported to play important role in actin reorganization, microtubule stability, cell cycle, motility, epithelial differentiation, and glucose uptake. Rac1 has also been reported to activate several protein kinases such as PAK-1, PI(4)P5-K, and thus is involved in cellular signaling cascades. Mutations in Rac1 have been recently linked to several cancers including melanoma and non-small cell lung cancer, making this protein a potential therapeutic target.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant full-length Rac1 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
Rac1 is a 192 amino acid protein with predicted molecular mass of ~ 21 kD.
Distribution

Tissue Distribution: Ubiquitously expressed

Cellular Distribution: Extracellular, plasma membrane, cytosol, endoplasmic reticulum, and Golgi apparatus

Function
Rac1 is activated by GTP binding and deactivated by GTP hydrolysis.
Interaction
Rac1 binds to PIP5K1A, RAB7a, SRGAP2, CYFIP1/SRA-1, PLXNB3, and PKN2.
Biology Area
Cancer Biomarkers, Cell Motility/Cytoskeleton/Structure, Cell Proliferation and Viability, Cell Structure, Neurodegeneration, Neuroinflammation, Neuroscience
Molecular Family
GTPases, Synaptic Vesicle Trafficking/Endocytosis
Antigen References
  1. Payapilly A, Malliri A. 2018. Curr. Opin. Cell. Biol. 2018.04.009.
  2. Melzer C, et al. 2017. Cell. Commun. Signal. s12964-017-0175-0.
  3. Zou T, et al. 2017. Clin. Genet. cge.12908.
Gene ID
19353 View all products for this Gene ID 363875 View all products for this Gene ID
UniProt
View information about Rac1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 07/09/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login / Register
Forgot your password? Reset password?
Create an Account