Purified anti-Rab8 Antibody

Pricing & Availability
Clone
A18073A (See other available formats)
Other Names
Rab8, Rab8A
Isotype
Mouse IgG2b, κ
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Product Citations
publications
A18073A_Purified_RAB8_Antibody_1_050619.png
Western blot of purified anti-Rab8 antibody (clone A18073A). Lane 1: Molecular weight marker; Lane 2: 20µg of human brain lysate; Lane 3: 20µg of mouse brain lysate; Lane 4: 20µg of rat brain lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A18073A_Purified_RAB8_Antibody_1_050619.png
    Western blot of purified anti-Rab8 antibody (clone A18073A). Lane 1: Molecular weight marker; Lane 2: 20µg of human brain lysate; Lane 3: 20µg of mouse brain lysate; Lane 4: 20µg of rat brain lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A18073A_Purified_RAB8_Antibody_2_050619.png
    ICC staining of purified anti-Rab8 antibody (clone A18073A) on HeLa cells. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 5 µg/ml of the primary antibody for overnight at 4°C, followed by incubation with 2.5 µg/ml of Alexa Fluor® 647 goat anti-mouse IgG (Cat. No. 405322) for one hour at room temperature. Flash Phalloidin™ Green 488 (Cat. No. 424201) was used to stain the F-actin cytoskeleton. The slide was mounted with fluoromount-G with DAPI.
Cat # Size Price Quantity Avail. Save
865401 25 µg £60
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865402 100 µg £158
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Description

Rab8 is a small GTP/GDP binding protein that belongs to the RAS superfamily. It is well established that Rab8 is an important participant of macroautophagy, and is essential for several apical transport pathways. Recruitment of Rab8a and TBK1 by the autophagy receptor optineurin initiates the autophagy process at specific protein aggregate site, which leads to the clearance of aggregated protein. Recent studies on C9ORF72, the gene that most commonly mutated in Amyotrophic Lateral Sclerosis and Frontotemporal Dementia (ALS-FTD), show that the C9ORF72/SMCR8/WDR41 complex acts as a GDP/GTP exchange factor for Rab8a, which facilitates the interaction between Rab8a and the autophagy receptor optineurin4.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human Rab8A
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1 - 5 µg per ml. For immunocytochemistry, a concentration range of 1 - 5 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone cross-react with Rab8A and Rab8B.

Antigen Details

Structure
Rab8 is a 207 amino acid protein with an apparent molecular mass of ~24 kD.
Distribution

Tissue Distribution: ubiquitously expressed
Cellular Distribution: Plasma membrane, cytoskeleton, cytosol, Golgi Apparatus, and endosome.

Function
Rab8 is a GTPase that plays an important role in intracellular membrane trafficking.
Interaction
MICALL1, MICALL2, Optineurin, MAP4K2
Cell Type
Astrocytes, Dopaminergic Neurons, GABAergic Neurons, Glutamatergic Neurons, Mature Neurons, Microglia, Neural Stem Cells, Neurons, Oligodendrocytes
Biology Area
Cancer Biomarkers, Cell Biology, Cell Cycle/DNA Replication, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation, Protein Trafficking and Clearance, Synaptic Biology
Molecular Family
Autophagosome Markers, Cytoskeletal Proteins, Organelle Markers, Tumor Suppressors
Antigen References
  1. Zhang X and Gao N. 2016. Small GTPases. 7:59
  2. Just WW and Peränen J. 2016. Biochim Biophys Acta. 1863:1006
  3. Ao X, et al. 2014. Cell Death Differ. 21(3):348
  4. Corbier C and Sellier C. 2017. Small GTPases. 8:181
Gene ID
4218 View all products for this Gene ID 17274 View all products for this Gene ID 117103 View all products for this Gene ID
UniProt
View information about RAB8 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05/06/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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