Purified anti-PPT1 Antibody

Pricing & Availability
Clone
O91F10 (See other available formats)
Other Names
PPT1, Palmitoyl-Protein Thioesterase 1
Isotype
Mouse IgG1, κ
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Product Citations
publications
O91F10_PURE_PPT1_Antibody_101118
Western blot of purified anti-PPT1 antibody (clone O91F10). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • O91F10_PURE_PPT1_Antibody_101118
    Western blot of purified anti-PPT1 antibody (clone O91F10). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Avail. Save
855801 25 µg £60
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855802 100 µg £158
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Description

PPT1 (Palmitoyl-protein thioesterase-1) was originally discovered as the enzyme that catalyzes polypeptide depalmitoylation. During lysosomal degradation, PPT1 removes the thioester-linked palmitic acid from the modified cysteine of targeted protein, which is important to palmitoylated protein degradation. Deficits in PPT1 have been identified as the leading cause of infantile neuronal ceroid liposuscinosis. Recent research has also linked PPT1 to Huntington Disease, Alzheimer disease, Schizophrenia, and mental retardation, as S-palmitoylation has been found to affect synaptic regulation by regulating AMPA receptors trafficking and function.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant protein corresponding to amino acids 100-306 of human PPT1.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2783442 (BioLegend Cat. No. 855801)
AB_2783443 (BioLegend Cat. No. 855802)

Antigen Details

Structure
PPT1 is a 306 amino acid protein with an apparent molecular mass of ~34 kD.
Distribution

Tissue Distribution: Ubiquitously expressed.

Cellular Distribution: Extracellular, nucleus, lysosome, golgi apparatus, and cytosol.

Function
PPT1 is involved in protein depalmitoylation and lysosomal degradation of palmitoylated protein.
Interaction
PPT1 interacts with CLN5, ATP5F1A, and ATP5F1B.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance, Synaptic Biology
Molecular Family
Presynaptic proteins
Antigen References
  1. Han J, et al. 2015. Acta. Pharm. Sin. B. 5:1-7.
  2. Hawkins-Salsbury JA, et al. 2013. Biochim. Biophys. Acta. 11:1906-9.
  3. Young FB, et al. 2012. Prog. Neurobiol. 97:220.
Gene ID
5538 View all products for this Gene ID
UniProt
View information about PPT1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/12/2018

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