Purified anti-PPP5 Antibody

Pricing & Availability
Clone
O95G5 (See other available formats)
Other Names
Protein Phosphatase 5 Catalytic Subunit, Serine/Threonine-Protein Phosphatase 5, Protein Phosphatase T, PP5
Isotype
Mouse, IgG1
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Product Citations
publications
O95G5_PURE_PPP5_Antibody_1_061719
Whole cell extracts (15 µg protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-PPP5 Antibody, clone O95G5, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
  • O95G5_PURE_PPP5_Antibody_1_061719
    Whole cell extracts (15 µg protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-PPP5 Antibody, clone O95G5, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
  • O95G5_PURE_PPP5_Antibody_2_061719
    Whole cell extracts (300 µg total protein) prepared from HeLa cells were immunoprecipitated overnight with 2.5 µg of Purified Mouse IgG1, κ Isotype Control Antibody (Cat. No. 400102) or Purified anti-PPP5 Antibody, clone O95G5. The resulting IP fractions and whole cell extract input (5%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with a 1.0 µg/mL (1:500 dilution) of O95G5. Lane M: Molecular Weight marker.
Cat # Size Price Quantity Avail. Save
622501 25 µg £57
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622502 100 µg £144
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Description

Protein phosphatase 5 catalytic subunit (PPP5) is a member of the phosphoprotein phosphatase family of enzymes and shows specificity towards phosphorylated serine and threonine residues. PPP5 displays broad substrate recognition and regulates diverse signal transduction cascades, including hormone and stress response pathways. PPP5 contains an autoinhibitory tetratricopeptide repeat (TPR) domain; PPP5 activity is augmented by Hsp90A, which releases TPR inhibition of the enzyme and also promotes the interaction of the PPP5 catalytic domain with client proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length recombinant human PP5 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. For immunoprecipitation, the suggested use of this reagent is 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone weakly reacts with two unidentified proteins of ~45 and 50 kD. This protein was also detected in O95G5 immunoprecipitates from HeLa extracts. We therefore do not recommend using this clone for co-immunoprecipitations.

Antigen Details

Structure
PPP5 is a 499 amino acid protein with a predicted molecular weight of 57 kD.
Distribution

Nucleus, cytosol, plasma membrane/ubiquitously expressed

Function
Phosphatase enzyme
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Proliferation and Viability, DNA Repair/Replication
Molecular Family
Protein Kinases/Phosphatase
Antigen References
  1. Wechsler T, et al. 2004. Proc Natl Acad Sci USA. 101:1247.
  2. Swingle MR, et al. 2004. J Biol Chem. 279:33992.
  3. Oberoji J, et al. 2016. Proc Natl Acad Sci USA. 113:9009.
Gene ID
5536 View all products for this Gene ID
UniProt
View information about PPP5 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 06/17/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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