Purified anti-PP2C-α Antibody

Pricing & Availability
Clone
P87F12H6 (See other available formats)
Other Names
PPM1A, Protein Phosphatase 1A, Protein Phosphatase 2C, Alpha isoform, Protein Phosphatase, Mg2+/Mn2+ Dependent 1A
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Product Citations
publications
P87F12H6_PURE_PP2C-alpha_Antibody_052019.png
Whole cell extracts (15 µg protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.1 µg/mL (1:5000 dilution) of purified anti-PP2C-α antibody, clone P87F12H6, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
  • P87F12H6_PURE_PP2C-alpha_Antibody_052019.png
    Whole cell extracts (15 µg protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.1 µg/mL (1:5000 dilution) of purified anti-PP2C-α antibody, clone P87F12H6, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
Cat # Size Price Quantity Avail. Save
617401 25 µg £57
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617402 100 µg £144
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Description

Protein Phosphatase 2Cα (PP2C-α) is a ubiquitously expressed magnesium- and manganese-dependent enzyme with a broad specificity of target substrates. It functions by dephosphorylating serine/threonine residues on its target proteins, and has been shown to regulate SMAD, PP2C-α, MAPK, p38 and JNK kinase signaling pathways. PP2C-α deficiency is associated with pancreatic and prostate cancers.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial recombinant human PPC2-α protein corresponding to amino acid residues 202-382.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

When tested for western blot application, this clone produced a non-specific band of roughly ~48 kD in certain cell lines.

Antigen Details

Structure
PP2C-α is a 382 amino acid protein with a predicted molecular weight of 42.4 kD.
Distribution

Cytosolic distribution/ubiquitously expressed.

Biology Area
DNA Repair/Replication, Signal Transduction
Molecular Family
Protein Kinases/Phosphatase
Antigen References
  1. Schilling SH, et al. 2006. Cell. 125:838.
  2. Lin X, et al. 2006. Cell. 125:915.
  3. Chau JF, et al. 2012. Nat. Commun. 3:836.
Gene ID
5494 View all products for this Gene ID
UniProt
View information about PP2C-alpha on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05/20/2019

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