Purified anti-PLCγ1 Phospho (Tyr783) Antibody

Pricing & Availability
Clone
A17025A (See other available formats)
Other Names
PLC1, NCKAP3, PLC-II, PLC148
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A17025A_Purified_PLCy1_Antibody_1_052119
Whole cell extracts (15 µg protein) from serum starved Jurkat cells untreated (-) or treated (+) with 5 mM H2O2 were resolved on a 4-12% Bis-Tris gel, transferred to nitrocellulose and probed with 0.25 µg/mL (1:2000 dilution) of purified anti-PLCγ1 Phospho (Tyr783) Antibody, clone A17025A, for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Equal PLCγ1 loading was confirmed by probing membranes with an anti-pan PLCγ1 antibody, and Direct-Blot™ HRP anti-β-Actin Antibody (Cat. No. 664804) was used as a loading control at a 1:25000 dilution. Lane M: Molecular Weight marker.
  • A17025A_Purified_PLCy1_Antibody_1_052119
    Whole cell extracts (15 µg protein) from serum starved Jurkat cells untreated (-) or treated (+) with 5 mM H2O2 were resolved on a 4-12% Bis-Tris gel, transferred to nitrocellulose and probed with 0.25 µg/mL (1:2000 dilution) of purified anti-PLCγ1 Phospho (Tyr783) Antibody, clone A17025A, for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Equal PLCγ1 loading was confirmed by probing membranes with an anti-pan PLCγ1 antibody, and Direct-Blot™ HRP anti-β-Actin Antibody (Cat. No. 664804) was used as a loading control at a 1:25000 dilution. Lane M: Molecular Weight marker.
  • A17025A_Purified_PLCy1_Antibody_2_052119
    Whole cell extracts (15 µg protein) from serum starved EL4 cells untreated (-) or treated (+) with 5 mM H2O2 were resolved on a 4-12% Bis-Tris gel, transferred to nitrocellulose and probed with 0.1 µg/mL (1:5000 dilution) of purified anti-PLCγ1 Phospho (Tyr783) Antibody, clone A17025A, for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Equal PLCγ1 loading was confirmed by probing membranes with an anti-pan PLCγ1 antibody, and Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution. Lane M: Molecular Weight marker.
  • A17025A_Purified_PLCy1_Antibody_3_060319
    Human peripheral blood lymphocytes were treated with (left), or without (right) Hydrogen Peroxide for 5 minutes, fixed with Fixation Buffer (Cat. No. 420801), permeabilized with True-Phos™ Perm Buffer (Cat. No. 425401), then intracellularly stained with purified anti-PLCγ1 Phospho (Tyr783) (clone A17025A), followed by by anti-mouse IgG PE, then stained with CD20 FITC.
  • A17025A_Purified_PLCy1_Antibody_4_060319
    Human T lymphoblastic leukemia cell line, Jurkat, was stimulated with (filled histogram) or without (open histogram) Hydrogen Peroxide for 5 minutes, fixed with Fixation Buffer (Cat. No. 420801), permeabilized with True-Phos™ Perm Buffer (Cat. No. 425401), and intracellularly stained with purified anti-PLCγ1 Phospho (Tyr783) (clone A17025A), followed by anti-mouse IgG PE.
Cat # Size Price Quantity Avail. Save
612401 25 µg £64
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612402 100 µg £151
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Description

Phospholipase C (PLC) enzymes cleave the phospholipid phosphatidylinositol 4,5-bisphosphate (PIP2) into second messenger molecules inositol 1,4,5-triposphate (IP3) and diacylglycerol (DAG) in response to extracellular stimuli. IP3 causes the release of calcium from the endoplasmic reticulum to begin an intracellular signaling cascade, while DAG activates Protein Kinase C.

There are four families of PLCs. PLC gamma (PLCγ) uniquely contains SRC domains SH2 and SH3. Receptor tyrosine kinases interact with these SRC domains to enzymatically activate PLCγ1 via phosphorylation at tyrosine 783. PLCγ1 is essential for cell proliferation, differentiation, and motility.  Overexpression or activation of PLCγ1 has been implicated in breast and prostate cancers as well as cancer cell invasion.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide from human PLCγ1 phosphorylated at Tyr783 emulsified in CFA.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICFC - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/ml. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.03 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
1290 and 1291 amino acid isoforms with predicted molecular weights of 148.5 and 148.7 kD, respectively
Function
T cell receptor signaling
Cell Type
T cells
Biology Area
Cell Proliferation and Viability, Signal Transduction
Molecular Family
Phospho-Proteins, Protein Kinases/Phosphatase
Antigen References
  1. Brady-Kalnay, et al. 2011. J Cell Biochem. 112:39.
  2. Schlessinger, et al. 2009. Cell. 138:514.
  3. Harden, et al. 2012. Subcell Biochem. 58:61.
  4. Wang Z, et al. 1998. Mol Cell Biol. 18:590.
  5. Rhee, et al. 1991. Cell. 65:435.
Gene ID
5335 View all products for this Gene ID
UniProt
View information about PLCgamma1 Phospho on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06/03/2019

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