Purified anti-PLAP Antibody

Pricing & Availability
Clone
8A11-C11-C9 (See other available formats)
Other Names
ALPP, PLAA, , Placental Alkaline Phosphatase 1
Isotype
Mouse IgG1, κ
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Product Citations
publications
8A11-C11-C9_PURE_PLAP_Antibody_1_062619
IHC staining of purified anti-PLAP antibody (clone 8A11-C11-C9) on formalin-fixed paraffin-embedded human placenta tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 8A11-C11-C9_PURE_PLAP_Antibody_1_062619
    IHC staining of purified anti-PLAP antibody (clone 8A11-C11-C9) on formalin-fixed paraffin-embedded human placenta tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 8A11-C11-C9_PURE_PLAP_Antibody_2_062619
    Western blot of purified anti-PLAP antibody (clone 8A11-C11-C9). Lane 1: Molecular weight marker; Lane 2: 20 µg of human placenta lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Avail. Save
866901 25 µg £60
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866902 100 µg £158
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Description

PLAP is a membrane-bound glycosylated dimeric enzyme that was initially identified in the placenta. PLAP is also known as phospholipase A2 activating protein. Abnormal amyloid beta-induced activation of synaptic phospholipase A2 results in synaptic degeneration due to elevated levels of platelet-activating factor, suggesting that PLAP may be a contributing factor in synaptic damage observed in Alzheimer’s disease patients. Clinically, PLAP is broadly used as a tumor marker, especially in geminoma, seminoma, and ovarian cancer.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
WB - Validated

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 2.0 - 10 µg/ml is suggested. For Western blotting, the suggested use of this reagent is 0.5 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
PLAP is a 795 amino acid protein with predicted molecular mass of ~87 kD and observed molecular mass of ~70 kD.
Distribution

Tissue Distribution: Ubiquitously expressed
Cellular Distribution: Plasma membrane

Function
Phospholipase A2 activating protein that may be involved in amyloid beta-induced synaptic degeneration.
Interaction
Ubiquitin, UBXN6, VCP, YOD1
Cell Type
Astrocytes, Endothelial cells, Neurons
Biology Area
Cancer Biomarkers, Neurodegeneration, Neuroscience, Synaptic Biology
Molecular Family
Protein Kinases/Phosphatase
Antigen References
  1. Watanabe S et al., 2012. Pediatr Neurosurg. 48(3):141
  2. Bate C et al., 2010. Mol Neurodegener. 5:13
  3. Huynh ML et al., 2011. Genesis. 49(11):851
Gene ID
18786 View all products for this Gene ID
UniProt
View information about PLAP on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 07/15/2019

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