Purified anti-PKCι Antibody

Pricing & Availability
Clone
W18205A (See other available formats)
Other Names
Protein Kinase C iota, Atypical Protein Kinase C-lambda/iota, Protein Kinase C iota Type, PRKC-lambda/iota, aPKC-lambda/iota, atypical protein kinase C, aPKC, aPKCλ/ι
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W18205A_PURE_PKCi_Antibody_1_103019
Whole cell extracts (15 µg protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-PKCι antibody (clone W18205A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker. Cell lines were loaded in order of ascending PRKCI expression; predicted expression data obtained from Human Protein Atlas.
  • W18205A_PURE_PKCi_Antibody_1_103019
    Whole cell extracts (15 µg protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-PKCι antibody (clone W18205A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker. Cell lines were loaded in order of ascending PRKCI expression; predicted expression data obtained from Human Protein Atlas.
  • W18205A_PURE_PKCi_Antibody_2_103019
    Whole cell extracts (250 µg total protein) prepared from HeLa cells were immunoprecipitated overnight with 2.5 µg of Purified Rat IgG2a, κ Isotype Ctrl Antibody (Cat. No. 400602) or purified anti-PKCι antibody (clone W18205A). The resulting IP fractions and whole cell extract input (10%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of clone W18205A. The secondary step was performed using light-chain-specific secondary antibodies. Lane M: Molecular weight marker.
Cat # Size Price Quantity Avail. Save
671201 25 µg £58
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671202 100 µg £147
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Description

Members of the protein kinase c family (PKC) are serine/threonine protein kinases with a broad set of substrates that can regulate diverse cellular processes. PKCι, also referred to as PKCι/λ, is a member of the atypical subfamily of PKC, which is characterized by complete independence of calcium and diacylglycerol as second messengers. It has been shown to play a role in cancer survival through multiple mechanisms: a. phosphorylation of the DNA-binding domain of FoxO1 resulting in transcriptional reprogramming of c-myc, which leads to increased proliferation of angiosarcoma cells, b. regulating membrane trafficking of type 1-matrix metalloprotease from late endosomal compartments to the plasma membrane, and c. mediating pro-survival by phosphorylating and inhibiting the pro-apoptotic factor BAD in glioblastomas. PKCι is also required for resistance to anti-apoptotic drugs in BCR-Abl-dependent leukemia and may represent a viable target for combinatorial therapies.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human PKCι protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone was tested for ICC using HeLa cells fixed with 4% PFA and permeabilized with methanol or Triton X-100; it failed due to weak staining.

Antigen Details

Structure
PKCι is a 596 amino acid protein with a predicted molecular weight of 68 kD.
Distribution

Cytosol and Nucleus/Ubiquitously expressed

Function
Protein Kinase/Cell Signaling
Biology Area
Cell Biology
Molecular Family
Protein Kinases/Phosphatase
Antigen References
  1. Nayak RC, et al. 2019. Nat. Commun. 10:46.
  2. Riddell M, et al. 2018. Nat. Commun. 9:5357.
  3. Rosse C, et al. 2014. Proc Natl Acad Sci U S A. 111:E1872.
  4. Desai S, et al. 2011. Biochim Biophys Acta. 1813:1190.
  5. Ishiguro H, et al. 2009. Proc Natl Acad Sci U S A. 106:16369.
Gene ID
5584 View all products for this Gene ID
UniProt
View information about PKCiota on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/30/2019

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