Purified anti-PINK1 Antibody

Pricing & Availability
Clone
DU46-1.1 (See other available formats)
Other Names
PTEN induced putative kinase 1, BRPK, PARK6, Serine/threonine-protein kinase PINK1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
DU46dash1point1_PURE_PINK1_Antibody_1_IHC_032416
IHC staining of clone DU46-1.1 on formalin-fixed paraffin-embedded Parkinson's disease brain tissue. Following antigen retrieval using sodium citrate pH 6, the tissue was incubated with DU46-1.1 at 10 µg/ml for 24 hours at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB) was used for detection with DAB chromogen according to kit instructions.
  • DU46dash1point1_PURE_PINK1_Antibody_1_IHC_032416
    IHC staining of clone DU46-1.1 on formalin-fixed paraffin-embedded Parkinson's disease brain tissue. Following antigen retrieval using sodium citrate pH 6, the tissue was incubated with DU46-1.1 at 10 µg/ml for 24 hours at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB) was used for detection with DAB chromogen according to kit instructions.
  • DU46dash1point1_PURE_PINK1_Antibody_2_WB_032416
    Western blot of clone DU46-1.1. Lane 1: Molecular weight marker; Lane 2: PINK1 transfected 293T cell lysate; Lane 3: Control 293T cell lysate. The blot was incubated with clone DU46-1.1 at 1 µg/ml, overnight at 4°C, followed by detection with fluorescently labeled goat anti-mouse secondary antibody.
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Cat # Size Price Quantity Avail. Save
846201 25 µg £60
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846202 100 µg £151
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Description

PINK1 (PTEN-induced putative kinase 1) is a 63 kD mitochondrial transmembrane protein that is often cleaved by PARL into a 53 kD fragment. The protein is involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). PINK1 phosphorylation of mitochondrial proteins can protect against mitochondrial dysfunction due to cellular stresses. PINK1 mutations can cause autosomal recessive early-onset Parkinson disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
WB, Direct ELISA - Validated
IP - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemcical staining. For immunohistochemistry, a concentration range of 1 - 10 µg/ml is recommended. For Western blotting, the suggested use of this reagent is 1 - 10 µg/ml. For Direct ELISA, the suggested use of this reagent is 0.02 - 2 µg/ml.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1.
 

This antibody recognizes PINK1.

Application References

(PubMed link indicates BioLegend citation)
  1. Lai YC, et al. 2015. EMBO. 34:2840. (WB, IP)
RRID
AB_2783414 (BioLegend Cat. No. 846201)
AB_2572127 (BioLegend Cat. No. 846202)

Antigen Details

Structure
PINK1 is a 581 amino acid, 63 kD, transmembrane protein that contains an N-terminal mitochondrial localization sequence, a serine/threonine kinase domain, and a C-terminal regulatory domain. PINK1 is proteolytically cleaved by PARL into a 55 kD fragment.
Distribution

Mitochondria and cytoplasm.

Function
PINK1 is involved in the clearance of damaged mitochondria via selective autophagy (mitophagy) by mediating activation and translocation of Parkin protein (PARK2). PARL-cleaved PINK1 is a cytoplasmic probe for damaged mitochondria. PINK1 phosphorylation of mitochondrial proteins can protect against mitochondrial dysfunction due to cellular stresses.
Interaction
PARKIN, Beclin 1, FBX07, DJ-1, HSP90/CDC37.
Ligand Receptor
PARKIN, Beclin 1, FBX07, DJ-1, HSP90/CDC37.
Biology Area
Cell Biology, Mitochondrial Function, Neurodegeneration, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance
Molecular Family
Mitochondrial Markers
Antigen References

1. Trempe JF, Fon EA. 2013. Front Neurol. 4:38.
2. Durcan TM, Fon EA. 2015. Genes Dev. 29(10):989.

Gene ID
65018 View all products for this Gene ID

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 03/25/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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