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Purified anti-PIK3R1 Antibody

Pricing & Availability
Clone
W16101A (See other available formats)
Regulatory Status
RUO
Other Names
Phosphatidylinositol 3-kinase regulatory subunit alpha, PI3-kinase subunit p85-alpha, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, GRB1,
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
1_W16101A_PURE_PIK3R1_Antibody_WB_051217
Total cell lysates (15µg protein) from Jurkat, HeLa (negative control) and NIH3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with 17µg/mL purified anti-PIK3R1 (clone W16101A) antibody and competitor’s antibody used at manufactures recommended concentration (upper). Proteins were visualized by chemiluminescence detection using a goat anti-rat-IgG secondary antibody conjugated to HRP for clone W16101A and a donkey anti-rabbit IgG Antibody conjugated to HRP for competitor’s antibody. Direct-Blot™ HRP anti-β-actin Antibody was used as a loading control (lower). Lane M: Molecular Weight ladder.
  • 1_W16101A_PURE_PIK3R1_Antibody_WB_051217
    Total cell lysates (15µg protein) from Jurkat, HeLa (negative control) and NIH3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with 17µg/mL purified anti-PIK3R1 (clone W16101A) antibody and competitor’s antibody used at manufactures recommended concentration (upper). Proteins were visualized by chemiluminescence detection using a goat anti-rat-IgG secondary antibody conjugated to HRP for clone W16101A and a donkey anti-rabbit IgG Antibody conjugated to HRP for competitor’s antibody. Direct-Blot™ HRP anti-β-actin Antibody was used as a loading control (lower). Lane M: Molecular Weight ladder.
  • 2_W16101A_PURE_PIK3R1_Antibody_ICC_031819
    NIH3T3 cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with 2 µg/ml anti-PIK3R1 antibody (clone W16101A) overnight at 4°C followed by Alexa Fluor® 594 (red) conjugated goat anti-rat IgG for one hour at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
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697102 100 µg £193
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Description

Phosphatidylinositol 3-kinase (PI3K) is a heterodimer comprised of a catalytic subunit encoded by PIK3CA (p110α) and one of a number of regulatory subunits. PIK3R1, also known as PI3K p85-alpha, is an 85kD regulatory subunit of PI3K. Upon activation of the receptor tyrosine kinases, PI3K phosphorylates inositol lipids to phosphatidylinositol-3,4,5-trisphosphate (PIP3), subsequently leads to activation of AKT and downstream effectors. The PI3K signaling regulates various cellular processes such as protein synthesis, cell growth, cell cycle progression, cell proliferation, angiogenesis, and survival. In addition, PI3K pathway has been found to play important roles in tumorigenesis and cancer progression. PIK3R1 appears to be a tumor suppressor due to its ability to stabilize the catalytic subunit of PI3K. Mutations in PIK3R1 have been associated with the autosomal dominant disorder SHORT syndrome.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human PIK3R1 recombinant protein (1-250 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified 
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 2.0 µg per ml. For immunocytochemistry, a concentration range of 0.5 - 5.0 µg per ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody may not react with the p85 beta based on sequence homology.

RRID
AB_2687110 (BioLegend Cat. No. 697102)

Antigen Details

Structure
724 amino acids with a predicted molecular weight of 83.6 kD. Contains an N-terminal SH3 domain, a Rho-GAP domain, and two C-terminal SH2 domains.
Distribution

Nucleus, cytoplasm, plasma membrane, ER membrane.

Function
PIK3R1 is a regulatory subunit of phosphatidylinositol 3-kinase and is involved in the signaling regulating protein synthesis, cell growth, cell cycle progression, cell proliferation, angiogenesis, and survival.
Interaction
Forms a heterodimer with the catalytic subunit of PIK3. Interacts with CSF1R, PIK3R2, XBP1, FER, TEK, PTK2, TOM1L1, LIME1, SOCS7, RUFY3, LYN, CBLB, IRS1, IRS4, FGR, HCK, ERBB4, and NTRK1.
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Protein Kinases/Phosphatase
Antigen References

1. Munkley J, et al. 2015. Oncoscience. 2:755.
2. Huang XP, et al. 2015. FEBS J. 282:579.
3. Wei Y, et al. 2013. Proc. Natl. Acad. Sci. USA. 110:6829.
4. Najib S, et al. 2012. Mol. Cell. Biol. 32:1004.
5. Anderson DH. 2010. Cell Cycle. 9:2055.
6. De Gregorio G, et al. 2007. Oncogene. 26:2039.

Gene ID
5295 View all products for this Gene ID
UniProt
View information about PIK3R1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05/15/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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