Purified anti-Peroxiredoxin 6 Antibody

Pricing & Availability
Clone
Poly28610 (See other available formats)
Other Names
PRDX6, peroxiredoxin VI, Peroxiredoxin-6, AOP2, Antioxidant protein 2
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
Poly28610_Purified_Peroxiredoxin-6_Antibody_1_090619
Western blot of purified anti-Peroxiredoxin 6 antibody (Poly28610). Lane 1: Molecular weight marker; Lane 2: 5 µg of human liver lysate; Lane 3: 5 µg of human brain lysate. The blot was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled donkey anti-rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28610_Purified_Peroxiredoxin-6_Antibody_1_090619
    Western blot of purified anti-Peroxiredoxin 6 antibody (Poly28610). Lane 1: Molecular weight marker; Lane 2: 5 µg of human liver lysate; Lane 3: 5 µg of human brain lysate. The blot was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled donkey anti-rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28610_Purified_Peroxiredoxin-6_Antibody_2_090619
    IHC staining of purified anti-Peroxiredoxin 6 antibody (Poly28610) on formalin-fixed paraffin-embedded human cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 0.1 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • Poly28610_Purified_Peroxiredoxin-6_Antibody_3_090619
    IHC staining of purified anti-Peroxiredoxin 6 antibody (Poly28610) on formalin-fixed paraffin-embedded human liver tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 0.1 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
Cat # Size Price Quantity Avail. Save
861001 25 µg £64
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Description

Human peroxiredoxin 6 is encoded by the PRDX6 gene and belongs to one of six peroxiredoxin families including PRDX1, PRDX2, PRDX3, PRDX4, PRDX5, and PRDX6. Peroxiredoxins are expressed ubiquitously and serve as thiol-specific antioxidant enzymes that regulate the redox state, and reduce H2O2 and lipid peroxides. Peroxiredoxin 6 interacts with saitohin (STH) which is nested within the intron 9 of the Tau gene demonstrates similar protein expression pattern as Tau protein. This interaction combined with abnormal expression of peroxiredoxin 6 may be a contributing factor to several neurodegenerative diseases including Alzheimer’s and Pick’s diseases. In Alzheimer's disease, peroxiredoxin 6 is upregulated in reactive astrocytes which are found colocalized with amyloid-beta (Aβ) peptide-positive diffuse plaques. This upregulation is believed to reduce oxidative stress caused by Aβ neurotoxicity.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Recombinant human PRDX6 (amino acid sequence Met1~Pro224)
Formulation
0.01 M PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 0.1 - 0.5 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody also detects peroxiredoxin 6 dimers in the liver lysate.

RRID
AB_2814585 (BioLegend Cat. No. 861001)

Antigen Details

Structure
Peroxiredoxin 6 is a 224 amino acid protein with predicted and observed molecular mass of ~25 kD.
Distribution

Tissue Distribution: Predominantly expressed in the lung, brain, liver, and testis
Cellular Distribution: Nucleus, cytosol, extracellular,  lysosome, and plasma membrane

Function
Peroxiredoxin 6 is involved in redox regulation and protects cells against lipid peroxidation.
Interaction
Peroxiredoxin 6 forms homodimer and heterodimer, and interacts with GSTP1, APEX1, and STH.
Cell Type
Astrocytes, Mature Neurons, Neurons
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Neurodegeneration, Neuroinflammation, Neuroscience, Signal Transduction
Molecular Family
APP/Aβ Degradation, Enzymes and Regulators, Lysosomal Markers
Antigen References
  1. Manevich Y, et al. 2014. Free Radic Biol Med. 72: 210.
  2. Zhou S, et al. 2016. Free Radic Biol Med. 94:145.
  3. Power JH, et al. 2008. Acta Neuropathol. 115:611.
Gene ID
9588 View all products for this Gene ID
UniProt
View information about Peroxiredoxin 6 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 10/03/2019

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