Purified anti-Peroxiredoxin 2 Antibody

Pricing & Availability
Clone
Poly28611 (See other available formats)
Other Names
PRDX2, Peroxiredoxin-2, Thioredoxin peroxidase 1
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
Poly28611_Purified_Peroxiredoxin-2_Antibody_1_090519
Western blot of purified anti-Peroxiredoxin 2 antibody (Poly28611). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate, Lane 3: 20 µg of mouse brain lysate, Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled donkey anti-rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28611_Purified_Peroxiredoxin-2_Antibody_1_090519
    Western blot of purified anti-Peroxiredoxin 2 antibody (Poly28611). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate, Lane 3: 20 µg of mouse brain lysate, Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled donkey anti-rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28611_Purified_Peroxiredoxin-2_Antibody_2_090519
    IHC staining of purified anti-Peroxiredoxin 2 antibody (Poly28611) on formalin-fixed paraffin-embedded human cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 0.5 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • Poly28611_Purified_Peroxiredoxin-2_Antibody_3_090519
    IHC staining of purified anti-Peroxiredoxin 2 antibody (Poly28611) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 0.5 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • Poly28611_Purified_Peroxiredoxin-2_Antibody_4_090519
    IHC staining of purified anti-Peroxiredoxin 2 antibody (Poly28611) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 0.5 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • Poly28611_Purified_Peroxiredoxin-2_Antibody_5_090519
    IHC staining of purified anti-peroxiredoxin 2 antibody (Poly28611) on formalin-fixed paraffin-embedded human cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 donkey anti-rabbit IgG for one hour at room temperature. The slide was mounted with Fluoromount-G with DAPI. The image was captured with a 40 objective. Scale bar: 50 µm
  • Poly28611_Purified_Peroxiredoxin-2_Antibody_6_090519
    IHC staining of purified anti-peroxiredoxin 2 antibody (Poly28611) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 donkey anti-rabbit IgG for one hour at room temperature. The slide was mounted with Fluoromount-G with DAPI. The image was captured with a 40 objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
861101 25 µg £64
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Description

Human peroxiredoxin 2 is encoded by the PRDX2 gene and belongs to one of six peroxiredoxin families including PRDX1, PRDX2, PRDX3, PRDX4, PRDX5, and PRDX6. Peroxiredoxins are expressed ubiquitously and serve as thiol-specific antioxidant enzymes that regulate the redox state, and reduce H2O2 and lipid peroxides. The antioxidative function of peroxiredoxin 2 protects cells from peroxide-induced apoptosis. Peroxiredoxin 2 is also involved in immune regulation. TNF-α- or lipopolysaccharides-induced peroxiredoxin 2 released by macrophages leads to IL-8 production and proinflammatory responses. In the post-ischemic brain, the extracellular release of peroxiredoxin 2 from necrotic brain cells elicits neuronal cell death mediated by macrophage TLR signaling.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Recombinant human PRDX2 (amino acid sequence Ala6~Tyr164)
Formulation
0.01 M PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 0.5 - 1.0 µg/ml for chromogenic staining and 1.0 - 5.0 µg/ml for fluorescent staining is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2814586 (BioLegend Cat. No. 861101)

Antigen Details

Structure
Peroxiredoxin 2 is a 198 amino acid protein with predicted and observed molecular mass of ~22 kD.
Distribution

Tissue Distribution: Predominantly expressed in the liver, lung, kidney, and brain
Cellular Distribution: Cytosol

Function
Peroxiredoxin 2 protects cells from oxidative stress and functions as a mediator of immune response.
Interaction
Peroxiredoxin 2 forms homodimers and a ring-like decamers. It also interacts with TIPIN.
Cell Type
Macrophages, Mature Neurons, Neurons
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Proliferation and Viability, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines, Enzymes and Regulators, Tumor Suppressors
Antigen References
  1. Hussain T, et al. 2016. Oxid Med Cell Longev. 7432797.
  2. Knoops B, et al. 2016. Mol Cells. 39:60.
  3. Sánchez-Font MF, et al. 2003. Cell Mol Life Sci. 60:1513.
Gene ID
7001 View all products for this Gene ID 21672 View all products for this Gene ID 29338 View all products for this Gene ID
UniProt
View information about Peroxiredoxin 2 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 10/03/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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