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Purified anti-PDPK1 Antibody

Pricing & Availability
Clone
W17215A (See other available formats)
Regulatory Status
RUO
Other Names
3-Phosphoinositide-Dependent Protein Kinase 1, PDK1
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W17215A_Purified_PDPK1_Antibody_051719.png
Whole cell extracts (15 µg protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-PDPK1 Antibody, clone W17215A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
  • W17215A_Purified_PDPK1_Antibody_051719.png
    Whole cell extracts (15 µg protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-PDPK1 Antibody, clone W17215A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
Cat # Size Price Quantity Check Availability Save
617601 25 µg £70
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617602 100 µg £174
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Description

3-Phosphoinositide-Dependent Protein Kinase 1 (PDPK1) is serine/threonine kinase that plays a central role in diverse cellular pathways. It functions by phosphorylating and activating members of the AGC family of serine kinases, including Akt1, Protein Kinases A and C, and p70 ribosomal protein S6 kinase. Loss or reduction of PDPK1 in the thymus results in defective T-cell differentiation and proliferative expansion.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human PDPK1 protein corresponding to amino acid residues 411-556
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone is predicted to recognize rat PDPK1 due to high sequence conservation between the immunizing protein and the rat homolog.

This clone is predicted to recognize PDPK1 isoforms 1-4 due to the presence of the immunizing sequence in each isoform.

This clone failed ICC and IP testing.

RRID
AB_2810673 (BioLegend Cat. No. 617601)
AB_2810674 (BioLegend Cat. No. 617602)

Antigen Details

Structure
PDPK1 is a 556 amino acid protein with a predicted molecular weight of 63 kDa. Four PDPK1 isoforms exist ranging in size from 48-63 kD.
Distribution

Peripheral plasma membrane protein/ubiquitously expressed

Function
Regulator of insulin, PKC, Akt1, and SMAD signal tranduction
Biology Area
Cell Biology
Molecular Family
Protein Kinases/Phosphatase
Antigen References
  1. Le Good JA, et al. 1998. Science. 281:2042.
  2. Dong LQ, et al. 2000. Proc. Natl. Acad. Sci. 97:5089.
  3. Hinton HJ, et al. 2004. Nat. Immunol. 5:539.
Gene ID
5170 View all products for this Gene ID
UniProt
View information about PDPK1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05/17/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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