Purified anti-PDHβ Antibody

Pricing & Availability
Clone
Poly28604 (See other available formats)
Other Names
PDHB, Pyruvate Dehydrogenase Beta
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
Poly28604_Purified_PDHB_Antibody_1_050119
Western blot of purified anti- PDHβ antibody (Poly28604). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 15 µg of rat brain lysate. The blot was incubated with 0.02 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled Donkey anti-Rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28604_Purified_PDHB_Antibody_1_050119
    Western blot of purified anti- PDHβ antibody (Poly28604). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 15 µg of rat brain lysate. The blot was incubated with 0.02 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled Donkey anti-Rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28604_Purified_PDHB_Antibody_2_050119
    IHC staining of purified anti- PDHβ antibody (Poly2860) on formalin-fixed paraffin-embedded human heart tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • Poly28604_Purified_PDHB_Antibody_3_050119
    IHC staining of purified anti- PDHβ antibody (Poly28604) on formalin-fixed paraffin-embedded mouse heart tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
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860401 25 µg £64
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Description

PDHβ is a component of the E1 pyruvate dehydrogenase (PDHE1). Two PDHβ subunits bind to two PDHα subunits to form the heterotetrameric PDHE1, which plays a role in the overall conversion of pyruvate to acetyl-Coa and CO2. Mutations in PDHβ cause one form of pyruvate dehydrogenase deficiency, which is clinically characterized by the buildup of lactic acid in the body, and a variety of neurological problems. Energy deficient young patients with severe PDH deficiency may have altered brain development, leading to epileptogenesis1.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Recombinant PDHb (Leu31~Asn341) with N-terminal His-Tag expressed in E.coli.
Formulation
0.01 M PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.02 - 0.1 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
PDHβ is a 359 amino acid protein with an apparent molecular mass of ~40 kD.
Distribution

Tissue Distribution: Ubiquitously expressed
Cellular Distribution: Mitochondrion and nucleus

Function
Beta subunit of the pyruvate dehydrogenase complex that catalyzes the conversion of pyruvate to acetyl-CoA and CO2.
Interaction
PDHβ forms heterotetramer with PDHα1 subunits.
Cell Type
Astrocytes, Mature Neurons, Microglia, Neural Stem Cells, Neurons, Oligodendrocytes
Biology Area
Cardiovascular Biology, Mitochondrial Function, Neurodegeneration, Neuroscience, Synaptic Biology
Molecular Family
Mitochondrial Markers
Antigen References
  1. Bhandary S, et al. 2015. Epilepsy Res. 116:40
  2. Prasad C, et al. 2011. Brain Dev. 33(10):856
Gene ID
5162 View all products for this Gene ID 68263 View all products for this Gene ID 289950 View all products for this Gene ID
UniProt
View information about PDHbeta on UniProt.org

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Go To Top Version: 1    Revision Date: 05/01/2019

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