Purified anti-Pax-2 Antibody (Previously Covance catalog# PRB-276P)

Pricing & Availability
Clone
Poly19010 (See other available formats)
Other Names
Paired box protein Pax-2, paired box homeotic gene 2, Opdc, optic disc coloboma
Previously
Covance Catalog# PRB-276P
Isotype
Rabbit Polyclonal IgG
Ave. Rating
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Product Citations
publications
1_Purified_Pax-2_Antibody_IHC_021315
Pax2 staining on normal human kidney tissue, demonstrating strong nuclear staining of epithelial cells in some renal tubules.
  • 1_Purified_Pax-2_Antibody_IHC_021315
    Pax2 staining on normal human kidney tissue, demonstrating strong nuclear staining of epithelial cells in some renal tubules.
  • 2_Poly19010_PURE_Pax-2_Antibody_2_WB_112817
    Western blot analysis of cell lysates from MCF-7 (low expression negative control) and Daudi (positive control) cells using Pax-2 rabbit primary antibody (Clone Poly19010,1:1000 dilution, 1 µg/ml) and HRP Donkey anti-rabbit secondary antibody (Cat. No. 406401, 1:3000 dilution). Direct-Blot™ HRP anti-GAPDH (Cat. No. 649203) was used as a loading control (1:1000 dilution).
Cat # Size Price Quantity Avail. Save
901002 25 µl £50
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901001 200 µl £212
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Description

Pax genes are expressed during embryogenesis in a temporally and spatially restricted manner in the developing neural tube. Pax-2 has been identified to be involved in kidney and optic nerve development. It belongs to the Pax group 2, including Pax-5 and Pax-8. Paired box (Pax) proteins are tissue specific transcription factors that contain a paired domain and typically a partial or complete homeodomain.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Chicken, Xenopus, Zebrafish
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
This Pax-2 antibody was generated against a recombinant protein containing 22kD of the Pax-2 sequence corresponding to amino acids 188-385.
Formulation
Phosphate-buffered solution + 0.03% Thimerosal.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

IHC - Quality tested
WB - Validated
IP - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining.

The optimal working dilution should be determined for each specific assay condition.
WB: 1-2 µg/ml (1:500-1:1000)
• Gel shifts: 1:10
IHC: 1:100

Tissue Sections: Formalin-fixed, paraffin-embedded tissues, acetone or PFA fixed frozen sections
Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as Retrieva-All 1 (Cat. No. 927901).
Incubation: 20 minutes at room temperature
Staining Localization: nuclear

Application Notes

This antibody is effective in immunoblotting (WB), immunohistochemistry (IHC), immunopurification and DNA band supershift experiments.

*Predicted MW = 44 kD
Positive Control Tissue: normal kidney or renal adenocarcinoma

This antibody recognizes Pax-2A and Pax-2B (two products of the Pax-2 gene). When linked to agarose beads, it has been used to immunoprecipitate DNA sequences recognized by Pax-2.


This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Hammes A, et al. 2001. Cell. 106:319.
  2. Phelps DE, Dressler GR. 1996. J Biol Chem. 271:7978.
  3. Rothenpieler UW, Dressler GR. 1993. Development. 119:711
  4. Dressler GR, et al. 1993. Nature. 362:65
  5. Dressler GR, Douglass EC. 1992. Proc Natl Acad Sci USA. 89:1179
  6. Puschel AW, et al. 1992. Mech Dev. 38:197
  7. Riley BB, et al. 1999. Development. 126:5669. (IHC)
Product Citations
  1. Huang L, et al. 2016. Neoplasia. 18:71-81. PubMed
  2. Zhao F, et al. 2016. Sci Rep. 6:23037. PubMed
  3. Prunskaite-Hyyryläinen R, et al. 2016. Hum Mol Genet. 25: 1059 - 1073. PubMed
  4. Ohnmacht J, et al. 2016. Development. 143: 1464 - 1474. PubMed
  5. Dauleh S, et al. 2016. PLoS One. 11: 0158997. PubMed
  6. Muscat A, et al. 2016. Clin Cancer Res. 22: 3560 - 3570. PubMed
  7. Ealy M, et al. 2016. Proc Natl Acad Sci U S A. 113: 8508 - 8513. PubMed
  8. Reginensi A, et al. 2016. Nat Commun. 7:12309. PubMed
  9. McNeill H, Reginensi A 2016. J Am Soc Nephrol. 10.1681/ASN.2016060611. PubMed
  10. Atsuta Y, Takahashi Y 2016. Development. 143: 3549 - 3559. PubMed
  11. C Tao, X Zhang, 2016. Cell Rep. 17:1832-1844. PubMed
  12. Taguchi A, et al. 2017. Cell Stem Cell. 21:730. PubMed
  13. Desgrange A, et al. 2017. Development. 144:4704. PubMed
  14. Hoshi M, et al. 2018. J Am Soc Nephrol. 29:775. PubMed
  15. O'Sullivan ML, et al. 2017. Glia. 65:1697. PubMed
  16. Mattei C, et al. 2019. Front Cell Dev Biol. 7:25. PubMed
  17. Kim E, et al. 2019. Sci Rep. 9:5025. PubMed
  18. Charlton–Perkins M, et al. 2019. Glia. 67:1401. PubMed
Publication Library
RRID
AB_2734656 (BioLegend Cat. No. 901002)
AB_2565001 (BioLegend Cat. No. 901001)

Antigen Details

Biology Area
Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology, Transcription Factors
Molecular Family
Nuclear Markers
Gene ID
5076 View all products for this Gene ID
UniProt
View information about Pax-2 on UniProt.org

Related FAQs

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Go To Top Version: 5    Revision Date: 11/28/2017

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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