Purified anti-PAK1 Antibody

Pricing & Availability
Clone
A17137A (See other available formats)
Other Names
PAK1, p21-activated kinase 1
Isotype
Mouse IgG2a, κ
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Product Citations
publications
A17137A_Pure_PAK1_Antibody_1_110118
Western blot of purified anti-PAK1 antibody (clone A17137A). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A17137A_Pure_PAK1_Antibody_1_110118
    Western blot of purified anti-PAK1 antibody (clone A17137A). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A17137A_Pure_PAK1_Antibody_2_110118
    IHC staining of purified anti-PAK1 antibody (clone A17137A) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • A17137A_Pure_PAK1_Antibody_3_110118
    IHC staining of purified anti-PAK1 antibody (clone A17137A) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • A17137A_Pure_PAK1_Antibody_4_110118
    IHC staining of purified anti-PAK1 antibody (clone A17137A) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
Cat # Size Price Quantity Avail. Save
856701 25 µg £60
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Description

PAK1 is a ubiquitously expressed protein that belongs to the group I PAK family of serine/threonine kinases, which are important mediators that link the Rho family GTPase signaling to cytoskeleton remodeling. PAK1 activation has been shown to play a role in tumorgenesis, actin dynamics, cell cycle progression, cell motility, and angiogenesis. The PAK family of kinases is affected in several brain diseases, including neurofibromatosis model of autism, Alzheimer’s Disease, Fragile X syndrome and schizophrenia1. Recently studies using electrophysiological recordings of hippocampal CA1 synaptic currents suggest that PAK1 plays a role in inhibitory synaptic transmission, where knocking out PAK affects the excitatory/inhibitory balance in the hippocampus, and may lead to dysregulation of synaptic strength.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 0.2 - 2.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2783462 (BioLegend Cat. No. 856701)

Antigen Details

Structure
PAK1 is a 545 amino acid protein with an apparent molecular mass of ~ 61 kD.
Distribution

Tissue distribution: Ubiquitously expressed in all tissues.

Cellular distribution: Cytosol, plasma membrane, and nucleus.

Function
PAK1 plays an important role in regulating cytoskeletal dynamics, spine morphology, and synaptic plasticity.
Interaction
Forms homodimer, NISCH, DVL1, MUSK, CDC2L1, CDC2L2, p110C, CDC42/P21, RAC1, DSCAM, PDPK1, RAF1, NCK1, NCK2
Cell Type
Astrocytes, Microglia, Neurons, Oligodendrocytes
Biology Area
Neuroscience, Synaptic Biology
Molecular Family
Postsynaptic proteins
Antigen References
  1. Xia S, et al. 2018. Small GTPases. 9(4):322
  2. Kreis P, et al. 2009. Cell Signal. 21(3):384
  3. Nikolic M. 2008. Mol Neurobiol. 37(2-3): 187
  4. Salminen A, et al. 2008. Biochem Biophys Res Commun. 371(4):587
Gene ID
5058 View all products for this Gene ID 18479 View all products for this Gene ID 29431 View all products for this Gene ID
UniProt
View information about PAK1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/02/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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