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Purified anti-Myelin Basic Protein Antibody

Pricing & Availability
Clone
SMI 94 (See other available formats)
Regulatory Status
RUO
Other Names
Golli-MBP, myelin A1 protein, myelin membrane encephalitogenic protein
Isotype
Mouse IgG1, κ
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Product Citations
publications
SMI9-4_Pure_Myelin-Basic-Protein_Antibody_1_123118
IHC staining of purified anti-Myelin Basic Protein antibody (clone SMI 94) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 100X (Cat. No. 927601), the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • SMI9-4_Pure_Myelin-Basic-Protein_Antibody_1_123118
    IHC staining of purified anti-Myelin Basic Protein antibody (clone SMI 94) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 100X (Cat. No. 927601), the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • SMI9-4_Pure_Myelin-Basic-Protein_Antibody_2_123118
    IHC staining of purified anti-Myelin Basic Protein antibody (clone SMI 94) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 100X (Cat. No. 927601), the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 10X objective. Scale bar: 50 µm
  • SMI9-4_Pure_Myelin-Basic-Protein_Antibody_3_123118
    IHC staining of purified anti-Myelin Basic Protein antibody (clone SMI 94) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 100X (Cat. No. 927601), the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • SMI9-4_Pure_Myelin-Basic-Protein_Antibody_4_123118
    Western blot of purified anti-Myelin Basic Protein antibody (clone SMI 94). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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836504 100 µg £189
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Description

Myelin basic protein (MBP) is a protein involved in the myelination of nerves in the central nervous system (CNS). MBP functions to maintain the correct structure of myelin, through its interaction with the lipids in the myelin membrane. The myelin sheath is made up of MBP and lipids, and acts as an insulator to increase the velocity of axonal impulse conduction. MBP plays a central role in the demyelinating disease multiple sclerosis (MS). A hallmark of the disease is the loss of the myelin sheath surrounding nerves, thought to be induced by antibodies against MBP.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
WB - Verified
ICC, IHC-F - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemcical staining. For immunohistochemistry, a concentration range of 1.0 - 5.0 μg/ml is suggested. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

SMI 94 reacts with the Myelin Basic Protein (MBP) peptic fragment 70-89 of the classic human myelin basic protein sequence.

SMI 94 detects developing and adult myelin, developing oligodendrocytes and distinguishes oligodendrocytes from astrocytes, microglia, neurons and other cells in brain sections. The combination of SMI 94 with SMI 91 and/or 99 is useful for immunocytochemical studies on the progression of normal and pathologic myelination.

Application References

(PubMed link indicates BioLegend citation)
  1. Kiryu-Seo S, et al. 2010. J. Neurosci. 30:6658. (ICC)
  2. Gensel JC, et al. 2009. J. Neurosci. 29:3956. (IHC-P)
  3. Wang H, et al. 2008. J. Cell. Biol. 182:1171. (ICC, WB)
  4. McLean NA, et al. 2014. PLoS One 9:e110174. (IHC-F)
  5. Tian C, et al. 2014. Front Cell Neurosci. 8:297. (IHC-P)
  6. Wüthrich C, et al. 2009. J. Neuropathol. Exp. Neurol. 68:15. (IHC, ICC)
  7. Maire CL, et al. 2014. Stem Cells 32:313. (IHC-P)
  8. Rusielewicz T, et. al. 2014. Glia 62:580. (ICC)
  9. Li C, et al. 2012. ASN Neuro. 4:e00102. (ICC)
  10. Huang JH, et al. 2009. Tissue Eng. Part A 15:1677. (IHC-P)
  11. Göttle P, et al. 2015. J. Neurosci. 35:906. (ICC)
  12. Zhao X, et al. 2010. Neuron. 65:612. (ICC)
Product Citations
  1. Munot P, et al. 2021. Neuropathol Appl Neurobiol. Online ahead of print. PubMed
  2. Wang S, et al. 2016. PLoS Genet. 12:e1006463. PubMed
  3. Mukherjee C, et al. 2020. Cell Metabolism. 32(2):259-272.e10. PubMed
  4. van der Veeken L, et al. 2020. Pediatr Res. 1011:87. PubMed
  5. Shi Y, et al. 2016. Mol Pain. 10.1177/1744806916656845. PubMed
  6. Carpena NT, et al. 2021. Int J Mol Sci. 22:. PubMed
  7. Skoff RP, et al. 2021. ASN Neuro. 13:17590914211009851. PubMed
  8. Southwood C, et al. 2016. J Neurosci. 36: 6803 - 6819. PubMed
  9. Reiche L, et al. 2021. Front Cell Neurosci. 15:653075. PubMed
  10. Murakami Y, et al. 2022. Nutrients. 14:. PubMed
  11. Wang Y, et al. 2019. Front Cell Neurosci. 13:241. PubMed
  12. Sánchez-de la Torre A, et al. 2022. Cell Death Dis. 13:585. PubMed
  13. Meyer Zu Reckendorf S, et al. 2022. J Neurosci. 42:2474. PubMed
  14. Mao Q, et al. 2019. Tissue Eng Part A. 25:887. PubMed
  15. Li T, et al. 2020. Cell Death Dis. 0.511805556. PubMed
  16. Aguado T, et al. 2021. Br J Pharmacol. 178:4176. PubMed
  17. Miyamoto Y, et al. 2016. Nat Commun. 7:13478. PubMed
  18. Li K et al. 2019. J Neurochem. 150(6):759-775 . PubMed
  19. Reed CB, et al. 2020. J Neurosci. 40:6165. PubMed
  20. Huang HT, et al. 2021. Scientific Reports. 11(1):6551. PubMed
  21. Huerga-Gómez A, et al. 2020. Glia. . PubMed
  22. Gravina G, et al. 2020. Front Immunol. 11:516. PubMed
  23. Sung HY et al. 2019. J Neurochem. 150(6):691-708 . PubMed
  24. Catanese A, et al. 2021. EMBO Mol Med. 13:e13131. PubMed
  25. Domènech-Estévez E, et al. 2016. J Neurosci. 36: 4506-4521. PubMed
  26. Rui Y, et al. 2020. Eneuro. 7:00. PubMed
RRID
AB_2616694 (BioLegend Cat. No. 836504)

Antigen Details

Structure
Myelin Basic Protein is a 304 amino acid protein with a molecular mass of 33kD.
Distribution

Cytoskeleton, plasma membrane, cytosol, and extracellular.

Function
Classic MBP isoforms (4-14) are the most abundant protein components of the myelin membrane in the CNS. They play a role in both its formation and stabilization. The non-classic isoforms may have a role in the early developing brain long before myelination.
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Gene ID
4155 View all products for this Gene ID
UniProt
View information about Myelin Basic Protein on UniProt.org

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Go To Top Version: 1    Revision Date: 12/31/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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