Loading...

Purified anti-Myelin Basic Protein, 119-131 Antibody

Pricing & Availability
Clone
BL28593 (See other available formats)
Regulatory Status
RUO
Other Names
Myelin basic protein, Golli-MBP, Myelin A1 protein, Myelin membrane encephalitogenic protein
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
BL28593_PURE_MBP119-131_Antibody_1_082119
Western blot of purified anti-Myelin Basic Protein, 119-131 antibody (clone BL28593). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of human brain membrane lysate; Lane 4: 10 ng of BSA-conjugated synthetic human MBP peptide (a.a 36-50); Lane 5: 10 ng of BSA-conjugated synthetic human MBP peptide (a.a 82-87); Lane 6: 10 ng of BSA-conjugated synthetic human MBP peptide (a.a 129-138); Lane 7: 10 ng of BSA-conjugated synthetic human MBP peptide (a.a 119-131). The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • BL28593_PURE_MBP119-131_Antibody_1_082119
    Western blot of purified anti-Myelin Basic Protein, 119-131 antibody (clone BL28593). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of human brain membrane lysate; Lane 4: 10 ng of BSA-conjugated synthetic human MBP peptide (a.a 36-50); Lane 5: 10 ng of BSA-conjugated synthetic human MBP peptide (a.a 82-87); Lane 6: 10 ng of BSA-conjugated synthetic human MBP peptide (a.a 129-138); Lane 7: 10 ng of BSA-conjugated synthetic human MBP peptide (a.a 119-131). The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Check Availability Save
859301 25 µg £62.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Myelin basic protein (MBP) is a protein involved in the myelination of nerves in the central nervous system (CNS). MBP functions to maintain the correct structure of myelin, through its interaction with the lipids in the myelin membrane. The myelin sheath is made up of MBP and lipids, and acts as an insulator to increase the velocity of axonal impulse conduction. MBP plays a central role in the demyelinating disease multiple sclerosis (MS). A hallmark of the disease is the loss of the myelin sheath surrounding nerves, thought to be induced by antibodies against MBP.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide corresponding to human MBP amino acids 191-131
Formulation
PBS, pH7.4 plus 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.05 - 0.2 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2814582 (BioLegend Cat. No. 859301)

Antigen Details

Structure
MBP is a 304 amino acid protein with predicted molecular mass of ~33 kD. MBP exists in multiple isoforms with observed molecular masses of ~ 18, 21 and 33 kD.
Distribution

Tissue Distribution: Central and peripheral nervous system
Cellular Distribution: Cytoskeleton, plasma membrane, cytosol, and extracellular

Function
Classic MBP isoforms (4-14) are the most abundant protein components of the myelin membrane in the CNS. They play a role in both its formation and stabilization. The non-classic isoforms may have a role in the early developing brain long before myelination.
Interaction
MBP forms homodimer
Cell Type
Oligodendrocytes
Biology Area
Cell Structure, Neurodegeneration, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Antigen References
  1. Llyasov AA, et al. 2018. Front Neurosci. 12:733
  2. Muller C, et al. 2013. Front Cell Neurosci. 7:169
  3. Harauz G, et al. 2013. J Neurochem. 125(3):334
Gene ID
4155 View all products for this Gene ID
UniProt
View information about Myelin Basic Protein 119-131 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08/21/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-Myelin Basic Protein, 119-131
ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account