Purified anti-mouse/rat CD62E (E-selectin) Antibody

Pricing & Availability
Clone
RME-1/CD62E (See other available formats)
Regulatory Status
RUO
Other Names
E-selectin, ELAM-1, LECAM-1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
RME-1-CD62E_Purified_CD62E_Antibody_1_111219
C57BL/6 mouse frozen kidney section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 5 µg/ml of purified anti-mouse CD62E (clone RME-1/CD62E) overnight at 4°C followed by 2.5µg of anti-mouse IgG Alexa Fluor® 488 (red) and Alexa Fluor® 594 anti-mouse CD326/Ep-CAM (clone G8.8) antibody (green) for 2 hours at room temper
  • RME-1-CD62E_Purified_CD62E_Antibody_1_111219
    C57BL/6 mouse frozen kidney section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 5 µg/ml of purified anti-mouse CD62E (clone RME-1/CD62E) overnight at 4°C followed by 2.5µg of anti-mouse IgG Alexa Fluor® 488 (red) and Alexa Fluor® 594 anti-mouse CD326/Ep-CAM (clone G8.8) antibody (green) for 2 hours at room temper
  • RME-1-CD62E_Purified_CD62E_Antibody_2_111219
    Mouse brain endothelial cell line bEND.3 cells were stimulated with TNF-α overnight. Then the cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes and blocked with 5% FBS for 30 minutes, subsequently stained with 5 µg/ml of purified anti-mouse/rat CD62E (clone RME-1/CD62E) (red) in blocking buffer for 3 hours at room temperature followed by DyLight™ 594 Goat anti-mouse IgG for another 2 hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with 40X objective.
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148801 50 µg £51.00
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148802 500 µg £188.00
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Description

CD62E (also known as E-selectin, ELAM-1, and LECAM-1) is a 115 kD type I transmembrane protein and a member of the selectin family. CD62E is highly expressed on activated endothelial cells. CD62E is involved in leukocyte tethering and rolling on activated endothelium at inflammatory sites and may also play a role in tumor metastasis and angiogenesis. CD62E binds to both Sialyl Lewis X (CD15s) and PSGL-1 (CD162).

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Rat E-selectin transfected chinese hamster ovary cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-F - Quality tested
ICC - Verified
WB, IP, ELISA, Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 2.5 - 5.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 5.0 - 10 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections4,5, Western blotting1, immunoprecipitation1, ELISA7, and blocking1,2,3. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 148803).

Application References

(PubMed link indicates BioLegend citation)
  1. Walter UM, et al. 1997. Hybridoma 16:249. (Block, WB, IP)
  2. Walter UM, et al. 1997. Immunology 92:290. (Block, Rat Reactivity)
  3. Hickey MJ, et al. 1998. Circ. Res. 83:1124. (Block, Mouse Reactivity)
  4. Bernardes-Silva M, et al. 2001. J. Cereb. Blood Flow Metab. 21:1115. (IHC)
  5. Alvarez A, et al. 2004. Blood 104:402. (IHC, Rat Reactivity)
  6. Piccio L, et al. 2002. J. Immunol. 168:1940. (IF)
  7. Walter UM, et al. 1997. in Hybridoma 16:355. (ELISA)
Product Citations
  1. King D, et al. 2019. Sci Rep. 9:4157. PubMed
RRID
AB_2564222 (BioLegend Cat. No. 148801)
AB_2564223 (BioLegend Cat. No. 148802)

Antigen Details

Structure
Member of the selectin family, 115 kD type I membrane protein; contains one C-type lectin domain and one EGF-like domain
Distribution

Highly expressed on activated endothelial cells

Function
Involved in leukocyte tethering and rolling on activated endothelium at inflammatory sites, may also play a role in tumor metastasis and angiogenesis
Ligand/Receptor
Sialyl Lewis X (CD15s) and PSGL-1 (CD162) bind to CD62E. Recently, CLA (cutaneous lymphocyte antigen) has also been reported to interact with CD62E
Cell Type
Endothelial cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Collins T, et al. 1991. J. Biol. Chem. 266:2466
  2. Bevilacqua MP, et al. 1987. Proc. Natl. Acad. Sci. USA 84:9238
  3. Berg EL, et al. 1991. J. Exp. Med. 174:1461.
  4. Lawrence MB, et al. 1993. J. Immunol. 151:6338.
  5. Walz G, et al. 1990. Science 250:1132
Gene ID
20351 View all products for this Gene ID
UniProt
View information about CD62E on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD62E Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse/rat CD62E (E-selectin) RME-1/CD62E IHC-F, ICC, WB, IP, ELISA, Block
Ultra-LEAF™ Purified anti-mouse/rat CD62E (E-selectin) RME-1/CD62E IHC-F, ICC, WB, IP, ELISA, Block
Go To Top Version: 5    Revision Date: 11/12/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-mouse/rat CD62E (E-selectin)

  • Ultra-LEAF™ Purified anti-mouse/rat CD62E (E-selectin)

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