Purified anti-mouse/rat CD49e Antibody

Pricing & Availability
HMα5-1 (See other available formats)
Other Names
α5 integrin, VLA-5 α chain, Integrin α5 chain, ITGA5
Armenian Hamster IgG
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Product Citations
C57BL/6 mouse bone marrow cells stained with purified HMα5-1, followed by anti-Armenian hamster IgG FITC
  • HMa5-1_Purified_090707
    C57BL/6 mouse bone marrow cells stained with purified HMα5-1, followed by anti-Armenian hamster IgG FITC
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103902 500 µg £147
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CD49e is a 135 kD protein, also known as α5 integrin or VLA-5 α chain. It is a member of the integrin family, expressed on thymocytes, pro-B cells, activated T cells, and mast cells. CD49e associates with CD29 (integrin β1 chain) to form the fibronectin receptor (VLA-5). CD49e plays a critical role in both adhesion and T cell costimulation. The primary ligand for CD49e/CD29 (VLA-5) is fibronectin. The HMα5-1 antibody has been shown to partially block CD49e mediated interactions. It has also been observed that plate-bound HMα5-1 antibody enhances IgE mediated degranulation and that in vivo injection of HMα5-1 antibody, in combination with anti-CD49d and anti-CD61 antibodies, blocks passive cutaneous anaphylaxis.

Product Details
Technical Data Sheet (pdf)

Product Details

Mouse, Rat
Antibody Type
Host Species
Armenian Hamster
Affinity-Purified mouse VLA-5 protein (α5β1, CD49e/CD29)
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.

FC - Quality tested
IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vivo inhibition of fibronectin receptor (VLA-5) functions and in vitro blocking of CD49e mediated interactions 1,2. The Ultra-LEAF™ Purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103909 & 103910).

Application References

(PubMed link indicates BioLegend citation)
  1. Yasuda M, et al. 1995. Int. Immunol. 7:251. (IP Block)
  2. Noto K, et al. 1995. Int. Immunol. 7:835. (Block)
  3. Lin JS, et al. 2010. J. Leukoc. Biol. 88:95. PubMed
Product Citations
  1. Lin J, et al. 2010. J Leukoc Biol. 88:95. PubMed
AB_313059 (BioLegend Cat. No. 103902)

Antigen Details

Integrin family, associates with integrin β1, 135 kD

Activated T cells, mast cells

Adhesion, costimulation
Cell Type
Mast cells, Mesenchymal Stem Cells, T cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kinashi T, et al. 1994 Blood Cells 20:25.
3. Hemler ME. 1990. Annu. Rev. Immunol. 8:365.

Gene ID
16402 View all products for this Gene ID 315346 View all products for this Gene ID
View information about CD49e on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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