Purified anti-mouse P2X7R Antibody

Pricing & Availability
1F11 (See other available formats)
Other Names
P2X7 receptor (P2X7R), P2RX7
Rat IgG2b, κ
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Product Citations
C57BL/6 splenocytes were stained with CD4 FITC and purified P2X7 receptor (clone 1F11, top) or rat IgG2b, κ isotype control (bottom) followed by biotinylated anti-rat IgG2b and SAv-PE.
  • 1F11_Purified_P2X7R_Antibody_FC_1_072814.jpg
    C57BL/6 splenocytes were stained with CD4 FITC and purified P2X7 receptor (clone 1F11, top) or rat IgG2b, κ isotype control (bottom) followed by biotinylated anti-rat IgG2b and SAv-PE.
  • 1F11_Purified_P2X7R_Antibody_FC_2_072814.jpg
Cat # Size Price Quantity Avail. Save
148702 100 µg £181
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P2X7R, also known as P2X7 receptor, belongs to the family of ligand-gated ion channel receptors. It is expressed on T cells, B cells, macrophages, and microglia. The receptor opens in the presence of extracellular ATP or NAD, leading to intracellular calcium mobilization. P2X7R activation requires higher concentrations of ATP compared to other P2X receptors. Longer stimulation results in larger pores, allowing passage of larger molecules. Activation of these molecules also leads to mitochondrial and cytoskeletal changes as well as IL-1β maturation and release. Ligation of P2X7 receptor can lead to membrane blebbing and cell death.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Host Species
Murine colon mast cells
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.

FC - Quality tested
IP, IHC, WB - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications for the relevant formats include: immunoprecipitation1, Western blotting1, immunohistochemistry1in vivo inhibition of intestinal inflammation and mast cell activation1.

Application References

(PubMed link indicates BioLegend citation)
  1. Kurashima Y, et al. 2012. Nat. Commun. 3:1034. (FC, IP, WB, IHC, FA)
AB_2564122 (BioLegend Cat. No. 148702)

Antigen Details

Ligand-gated ion channel, trimer - two transmembrane regions and one cytosolic region.

Immune cells, including mast cells, microglia, macrophages, T cells, and B cells.

Ion channel activated by extracellular ATP.
Cell Type
B cells, Macrophages, Mast cells, T cells, Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Antigen References

1. Surprenant A, et al. 1996. Science. 272:735.
2. Chused TM, et al. 1996. J. Immunol. 157:1371.
3. Gargett CE, et al. 1997. Br. J. Pharmacol. 122:911.
4. Kawamura H, et al. 2006. J. Immunol. 176:2152.
5. Pelegrin P. 2011. Br. J. Pharmacol. 163:908.
6. Bartlett R, et al. 2014. Pharmacol. Rev. 66:638.
7. Alves LA, et al. 2014. Biochim. Biophys. Acta. 1838:2578.

Gene ID
18439 View all products for this Gene ID
View information about P2X7R on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 08/13/2014

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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