Purified anti-mouse MIF Antibody

Pricing & Availability
Clone
M3907B02 (See other available formats)
Regulatory Status
RUO
Other Names
Macrophage migration inhibitory factor (MMIF), Glycosylation inhibiting factor (GIF), GLIF, Phenylpyruvate Tautomerase, L-Dopachrome Tautomerase, L-Dopachrome Isomerase
Isotype
Rat IgG2a, κ
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Product Citations
publications
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532502 500 µg £274.00
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Description

MIF (human macrophage migration inhibitory factor) is a non-glycosylated polypeptide expressed by multiple cell types, which include activated T cells, macrophages, eosinophils, epithelial cells, and endothelial cells. It is secreted by response to diverse inflammatory stimuli, and has been associated with a clear pro-inflammatory and proatherogenic role in multiple studies involving patients and animals. MIF also positively correlates with obesity as well as insulin resistance and contributes to adipose tissue inflammation by modulating ATM functions. Furthermore, MIF has been described as a protein that plays an essential role in both innate and acquired immunity.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
In-house recombinant mouse MIF.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration of 3.0 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of MIF recombinant protein ranging from 10 to 0.16 ng/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance.

Application Notes

ELISA Capture: The purified M3907B02 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5240 antibody (Cat. No. 524004) as the detecting antibody.

Note: For testing mouse MIF in serum, plasma, cell culture supernatant, and cell lysate, BioLegend's LEGEND MAX™ Kits are specially developed and recommended.

RRID
AB_2686958 (BioLegend Cat. No. 532502)

Antigen Details

Structure
115 amino acids with a predicted molecular weight of approximately 12.5 kD.
Distribution

Activated T-cells, macrophages, eosinophils, epithelial cells, and endothelial cells.

Function
MIF has been associated with a clear pro-inflammatory and proatherogenic role in cardiovascular diseases. MIF also plays important roles in adipose tissue inflammation and innate and acquired immunity.
Interaction
Monocytes and macrophages, mesenchymal cells, and B-cells.
Ligand/Receptor
CD44/CD74, CXCR4, CXCR2, and CXCR7.
Cell Type
T cells, Macrophages, Eosinophils, Epithelial cells, Endothelial cells
Biology Area
Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Innate Immunity, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References

1. Zhou H, et al. 2009. PLoS One. 4:e6087.
2. Potolicchio I, et al. 2003. J. Biol. Chem. 278:30889.
3. Meyer-Siegler K and Iczkowski K. 2005. BMC Cancer 5:73.
4. Babu SN, et al. 2012. Asian Pac. J. Cancer Prev. 13:1737.
5. van der Vorst EP, et al. 2015. Front Immunol. 6:373.
6. Kim BS, et al. 2015. Exp. Mol. Med. 47:e161.
7. Sanchez-Zamora YI, et al. 2014. J. Diabetes Res. 2014:804519.
8. Gilliver SC, et al. 2011. Exp. Dermatol. 20:1.
9. Bach JP, et al. 2008. Oncology 75:127.

Gene ID
17319 View all products for this Gene ID
UniProt
View information about MIF on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 04/10/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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