- 3D10 (See other available formats)
- Regulatory Status
- Other Names
- Klra8, Cmv1
- Mouse IgG1, κ
- Ave. Rating
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- Product Citations
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Ly49H, also known as Cmv1 and Klra8, is a 28.3 kD protein. It is expressed on NK cells (but not on NKT cells) from C57BL/6 mice (but not Balb/c or DBA/2 mice). Expression of Ly49H has been associated with resistance to MCMV infection in C57BL/6 mice. Modulation of Ly49H expression by its antibody made MCMV-resistant C57BL/6 mice susceptible to infection. Unlike Ly49A, Ly49H lacks the ITIM (immunoreceptor tyrosine-based inhibitory motif) but has ITAM (immunoreceptor tyrosine-based activation motif), which is associated with DAP-12 (KARAP, killer cell activating motif).Product Details
- Antibody Type
- Host Species
- C1498-Ly49A/H transfectant
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
IP, Block - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats of this clone) include: immunoprecipation1, and in vitro2 and in vivo3 blocking activity. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 144717 & 144718).
(PubMed link indicates BioLegend citation)
- Guseva NV, et al. 2010. Eur. J. Immunol. 40:2618. (IP)
- Orr MT, et al. 2009. J. Exp. Med. 206:807. (Block)
- Rodriguez M, et al. 2004. J. Immunol. 173:6312. (Block)
- Product Citations
AB_2561549 (BioLegend Cat. No. 144702)
- 28.3 kD
NK cells but not NKT cells
- Interacts with m157, a cytomegalovirus-encoded protein
- Cell Type
- NK cells
- Biology Area
- Immunology, Innate Immunity
- Antigen References
1. Gosselin P, et al. 1999. J. Leuko. Bio. 66:165.
2. Lee SH, et al. 2001. Nat. Genet. 28:42.
3. Daniels KA, et al. 2001. J. Exp. Med. 194:29.
- Gene ID
- 16639 View all products for this Gene ID
- View information about Ly49H on UniProt.org
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