Purified anti-mouse Ly49D Antibody

Pricing & Availability
4E5 (See other available formats)
Regulatory Status
Other Names
Rat IgG2a, κ
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Product Citations
C57BL/6 splenocytes stained with CD49b (DX5) APC and purified 4E5 conjugated with FITC
  • 4E5_FITC_1_051710
    C57BL/6 splenocytes stained with CD49b (DX5) APC and purified 4E5 conjugated with FITC
  • 4E5_FITC_2_051710
    C57BL/6 splenocytes stained with CD49b (DX5) APC and rat IgG2a FITC isotype control
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138302 500 µg £201
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Ly49D is a type II transmembrane protein belonging to the Ly49 family of NK-cell receptors in the C-type lectin superfamily.  Ly49D is expressed on subsets of natural killer (NK) cells in C57BL/6, C3H/He and SJL mouse strains, but not Balb/c, DBA/2, AKR and CBA/J.  The receptors form homodimers that bind to MHC class I alloantigens.  Unlike inhibitory Ly49 family members, Ly49D lacks a cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM) but contains a cytoplasmic immunoreceptor tyrosine-based activation motif.  The activating receptor Ly49D delivers stimulatory signals for target cell lysis upon interacting with H2-Dd, Dr and Dsp2, but not with H2b or H2k class I antigens.  Cross-linking of Ly49D activates intracellular kinase activity and calcium mobilization in NK cells.  In 129/J mice, 4E5 antibody cross-reacts with Ly49O, LY49R and Ly49V. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.

FC - Quality tested
IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for relevant formats) include: immunoprecipitation1 and blocking2, 3 of CHO cell or Con A-activated lymphoblast killing by NK cells.

Application References

(PubMed link indicates BioLegend citation)
  1. Mason LH, et al. 1998. J. Immunol. 160:4148. (IP)
  2. Idris A, et al. 1999. Proc. Natl. Acad. Sci. 96:6330. (FC Block)
  3. George TC, et al. 1999. J. Immunol. 162:2035. (FC Block)
  4. Ortaldo JR, et al. 1999. J. Leukoc. Biol. 66:512. (FC)
  5. Ortaldo JR, et al. 1998. J. Immunol. 160:1158. (FC)
Product Citations
  1. Goh W, et al. 2020. Cell Rep. 33:108285. PubMed
AB_10574460 (BioLegend Cat. No. 138302)

Antigen Details

A type II transmembrane protein belonging to the Ly49 family of NK-cell receptors in the C-type lectin superfamily.

Subsets of natural killer (NK) cells in C57BL/6, C3H/He and SJL, but not Balb/c, DBA/2, AKR and CBA/J.

Plays an important role in activating natural killer (NK) cell cytolytic activity.
MHC class I alloantigens
Cell Type
NK cells
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Yokoyama WM and Seaman WE. 1993. Annu. Rev. Immunol. 11:613.
2. Takei F, et al. 1997. Immunol. Rev. 155:67.
3. Hanke T, et al. 1999. Immunity 11:67.
4. Mason LH, et al. 1996. J. Exp. Med. 184:2119.

Gene ID
16635 View all products for this Gene ID
View information about Ly49D on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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  • TotalSeq™-B0841 anti-mouse Ly49D


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