Purified anti-mouse IL-22 Antibody

Pricing & Availability
Clone
BL35175 (See other available formats)
Other Names
Interleukin-22, IL-TIF, IL-10-related T-cell-derived-inducible factor
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
BL35175_PURE_IL22_Antibody_WB_050416
Western blot analysis of 50 ng of recombinant human IL-22 (lane 1) and 50 ng of recombinant mouse IL-22 (lane 2) using purified anti-mouse IL-22 (clone BL35175) antibody.
  • BL35175_PURE_IL22_Antibody_WB_050416
    Western blot analysis of 50 ng of recombinant human IL-22 (lane 1) and 50 ng of recombinant mouse IL-22 (lane 2) using purified anti-mouse IL-22 (clone BL35175) antibody.
Cat # Size Price Quantity Avail. Save
517501 25 µg £71
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517502 100 µg £151
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Description

IL-22, a cytokine structurally related to IL-10, was originally identified in the mouse as a gene induced by IL-9 in T cells and mast cells. IL-22 was initially designated as IL-TIF (IL10-related T cell-derived inducible factor). Mouse IL-TIF consists of 179 amino acids, including four cysteins, and has a predicted molecular weight of 20 kD.  Biological activity of IL-22 is initiated by binding to a cell-surface complex composed of IL-22R1 and IL-10R2 receptor chains and further regulated by interactions with a soluble binding protein, IL-22BP, which shares sequence similarity with an extracellular region of IL-22R1 (sIL-22R1). Both chains of the IL-22R complex belong to the class II cytokine receptor families. Two types of IL-22-binding receptor have been discovered, a membrane-bound receptor and a soluble receptor, both encoded by different genes. IL-22 is produced by immune cells and acts on non-immune cells to regulate local tissue inflammation. As a product of the recently identified T helper 17 lineage of CD4(+) effector lymphocytes, IL-22 plays a critical role in mucosal immunity as well as in dysregulated inflammation observed in autoimmune diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse, Cross-Reactivity: Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mature recombinant protein mouse Interleukin-22 (Leu34-Val179)
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 0.5 µg/ml dilution buffer for each mini-gel.

Application Notes

Clone BL35175 has been shown to be useful for detecting mouse and human IL-22 by Western blotting.

Product Citations
  1. Ke Y, et al. 2011. J Immunol. 187:2130. PubMed
Publication Library
RRID
AB_10695475 (BioLegend Cat. No. 517501)
AB_10694243 (BioLegend Cat. No. 517502)

Antigen Details

Structure
179 aa with with a putative 33 aa signal peptide that is cleaved to generate a 147 aa mature protein.
Distribution
Secreted, Th17
Function
Cytokine that contributes to the inflammatory response in vivo.
Ligand/Receptor
Binds to IL-22 receptor complex that consists of two receptor subunits, IL22R and IL10Rβ.
Cell Type
Th17
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References

1. Dumoutier L, et al. 2000. J. Immunol. 164:1814.
2. Xie MH, et al. 2000. J. Biol. Chem. 275:31335.
3. Jones BC, et al. 2008. Structure 16:1333.
4. Sonnenberg GF, et al. 2010. J. Exp. Med. 207:1293.
5. Kotenko SV, et al. 2001. J. Immunol. 166:7096.
6. Chang C, et al. 2006. Cell Research 16:902.

Gene ID
50929 View all products for this Gene ID
UniProt
View information about IL-22 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 05/04/2016

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