Purified anti-mouse Ig light chain κ Antibody

Pricing & Availability
Clone
RMK-45 (See other available formats)
Other Names
Immunoglobulin light chain kappa
Isotype
Rat IgG
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RMK-45_Purified_Igk_Antibody_FC_1_041714
C57BL/6 splenocytes were stained with purified anti-mouse light chain Ig κ (clone RMK-45) (filled histogram) or purified rat IgG1, κ isotype control, followed by anti-rat IgG PE (opened histogram).
  • RMK-45_Purified_Igk_Antibody_FC_1_041714
    C57BL/6 splenocytes were stained with purified anti-mouse light chain Ig κ (clone RMK-45) (filled histogram) or purified rat IgG1, κ isotype control, followed by anti-rat IgG PE (opened histogram).
  • RMK-45_Purified_Igk_Antibody_FC_2_041714
    Human peripheral blood lymphocytes were stained with purified CD3 (clone UCHT1), and then stained with purified anti-mouse Ig light chain κ (clone RMK-45) (filled histogram) or purified rat IgG1, κ isotype control, followed by anti-rat IgG PE (opened histogram).
Cat # Size Price Quantity Avail. Save
409502 500 µg £97
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Description

The RMK-45 monoclonal antibody reacts with immunoglobulin light chain kappa in all tested mouse haplotype (Igh-a and b). It does not react with the lambda chain.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ELISA - Validated

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The RMK-12 monoclonal antibody may be used as a primary antibody for ELISA or as secondary for detection of mouse Ig kappa.

RRID
AB_2563297 (BioLegend Cat. No. 409502)

Antigen Details

Structure
Immunoglobulin family
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Protein Synthesis
Antigen References

1. Ludwig TE, et al. 2006. Nat. Methods. 3:637.
2. Nguyen DH, et al. 2005. J. Immunol. 175:228.
3. Bardor M, et al. 2005. J. Biol. Chem. 280:4228.
4. Diaz SL, et al. 2009. PLoS One. 4:e4241.

Gene ID
243469 View all products for this Gene ID
UniProt
View information about Ig light chain kappa on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 04/21/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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