- JKmNGF-1 (See other available formats)
- Other Names
- Nerve growth factor, NGF, β-NGF
- Mouse IgG1, κ
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- Product Citations
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Nerve growth factor (NGF) is a small secreted protein that is important for the growth, maintenance, and survival of certain target neurons (nerve cells). NGF consists of three types of subunits, α, β and γ, which specifically interact to form a 7S, 130,000 molecular weight complex. This complex contains two identical 118-amino acid β-chains, which are solely responsible for nerve growth stimulating activity of NGF. NGF is essential for the development and phenotypic maintenance of neurons in the peripheral nervous system (PNS) and for the functional integrity of cholinergic neurons in the central nervous system (CNS).Product Details
- Mouse, Human, 2.5S, 7S, and β-NGF forms.
- Antibody Type
- Host Species
- recombinant human NGF (a.a. 46-62)
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
ELISA Capture - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. For ELISA applications, a concentration range of 6 - 10 µg/ml is recommended. Additionally, BioLegend recommends the usage of Tris-Buffered Saline (TBS), pH = 7.2 for all washes and incubations, except substrate buffer. Do not use PBS as results may vary dramatically. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
ELISA Capture: The purified JKmNGF-1 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated JKhNGF-2 antibody (Cat. No. 509802) as the detecting antibody for detecting mouse NGF.
AB_315543 (BioLegend Cat. No. 509701)
AB_2282783 (BioLegend Cat. No. 509702)
- Kallikrein family of serine proteases; dimer; 13.5 kD (Mammalian)
- Activates JNK and NF-κB; involved in bone resorption; induces the expression of IL-1, IL-6, IL-12, IL-15 in dendritic cells
- Cell Sources
- Hypothalamus, pituitary, thyroid gland, testes, epididymis, vascular smooth muscle cells, fibroblasts, mast cells, eosinophils
- Cell Targets
- Neurons, B cells, mast cells, eosinophils
- Low-affinity NGF receptor (LNGFR, p75 LNGFR, fast NGF receptor); high-affinity NGF receptor (trkA)
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Antigen References
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Levi A, et al. 1991. Annu. Rev. Pharmacol. Toxicol. 31:205.
3. Varon S, et al. 1991. Adv. Exp. Med. Biol. 296:267.
4. Micera A, et al. 2003. Cytokine Growth Factor Rev. 14:369.
- Upregulated by glutamate, vitamin D3, IL-6, FGF-basic, astrocyte-specific IL-1, TNF-α, PDGF, TGF-β; downregulated by GABAergic neuronal activity, glucocorticoids, Schwann cell-specific TGF-β
- Gene ID
- 18049 View all products for this Gene ID 4803 View all products for this Gene ID
- View information about NGF on UniProt.org
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