Purified anti-mouse/human NGF Antibody

Pricing & Availability
Clone
JKmNGF-1 (See other available formats)
Other Names
Nerve growth factor, NGF, β-NGF
Isotype
Mouse IgG1, κ
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Cat # Size Price Quantity Avail. Save
509701 50 µg £60
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509702 500 µg £198
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Description

Nerve growth factor (NGF) is a small secreted protein that is important for the growth, maintenance, and survival of certain target neurons (nerve cells). NGF consists of three types of subunits, α, β and γ, which specifically interact to form a 7S, 130,000 molecular weight complex. This complex contains two identical 118-amino acid β-chains, which are solely responsible for nerve growth stimulating activity of NGF. NGF is essential for the development and phenotypic maintenance of neurons in the peripheral nervous system (PNS) and for the functional integrity of cholinergic neurons in the central nervous system (CNS).

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse, Human, 2.5S, 7S, and β-NGF forms.
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
recombinant human NGF (a.a. 46-62)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA applications, a concentration range of 6 - 10 µg/ml is recommended. Additionally, BioLegend recommends the usage of Tris-Buffered Saline (TBS), pH = 7.2 for all washes and incubations, except substrate buffer. Do not use PBS as results may vary dramatically. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Capture: The purified JKmNGF-1 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated JKhNGF-2 antibody (Cat. No. 509802) as the detecting antibody for detecting mouse NGF.

RRID
AB_315543 (BioLegend Cat. No. 509701)
AB_2282783 (BioLegend Cat. No. 509702)

Antigen Details

Structure
Kallikrein family of serine proteases; dimer; 13.5 kD (Mammalian)
Bioactivity
Activates JNK and NF-κB; involved in bone resorption; induces the expression of IL-1, IL-6, IL-12, IL-15 in dendritic cells
Cell Sources
Hypothalamus, pituitary, thyroid gland, testes, epididymis, vascular smooth muscle cells, fibroblasts, mast cells, eosinophils
Cell Targets
Neurons, B cells, mast cells, eosinophils
Receptors
Low-affinity NGF receptor (LNGFR, p75 LNGFR, fast NGF receptor); high-affinity NGF receptor (trkA)
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Levi A, et al. 1991. Annu. Rev. Pharmacol. Toxicol. 31:205.
3. Varon S, et al. 1991. Adv. Exp. Med. Biol. 296:267.
4. Micera A, et al. 2003. Cytokine Growth Factor Rev. 14:369.

Regulation
Upregulated by glutamate, vitamin D3, IL-6, FGF-basic, astrocyte-specific IL-1, TNF-α, PDGF, TGF-β; downregulated by GABAergic neuronal activity, glucocorticoids, Schwann cell-specific TGF-β
Gene ID
18049 View all products for this Gene ID 4803 View all products for this Gene ID
UniProt
View information about NGF on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 07/22/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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