Purified anti-mouse F4/80 Recombinant Antibody

Pricing & Availability
Clone
QA17A29 (See other available formats)
Other Names
EMR1, Ly71
Isotype
Mouse IgG1, κ
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Product Citations
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QA17A29_Purified_F4-80_Antibody_1_090619
Thioglycolate-elicited Balb/c mouse peritoneal macrophages were stained with purified mouse F4//80 (clone QA17A29, left) or mouse IgG1, κ isotype control (right), followed by anti-mouse IgG1 PE.
  • QA17A29_Purified_F4-80_Antibody_1_090619
    Thioglycolate-elicited Balb/c mouse peritoneal macrophages were stained with purified mouse F4//80 (clone QA17A29, left) or mouse IgG1, κ isotype control (right), followed by anti-mouse IgG1 PE.
Cat # Size Price Quantity Avail. Save
157301 50 µg £27
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157302 500 µg £148
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Description

F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8+ T cells-mediated peripheral tolerance.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
Murine macrophages
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2814089 (BioLegend Cat. No. 157301)

Antigen Details

Structure
A 160 kD glycoprotein and a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family.
Distribution

Expressed on the majority of tissue macrophages

Function
Required for induction of CD8+ T cells-mediated peripheral tolerance.
Cell Type
Macrophages, Monocytes
Biology Area
Immunology, Innate Immunity
Antigen References
  1. Schaller E, et al. 2002. Mol. Cell. Biol. 22:8035.
  2. Stevceva L, et al. 2001. BMC Clin Pathol. 1:3.
  3. Kobayashi M, et al.2008. J. Leukoc. Biol. 83:1354.
  4. Poeckel D, et al. 2009. J. Biol Chem. 284:21077.
  5. Glass AM, et al. 2013. J. Immunol. 190:4830.
  6. Koehm S, et al. 2007. J. Allergy Clin. Immunol. 120:570.
  7. Rankin AL, et al. 2010. J. Immunol. 184:1526.
  8. Sasi SP, et al. 2014. J Biol Chem. 289:14178. 
  9. Thakus VS, et al. 2014. Toxicol Lett. 230:322.
  10. Ohnishi A, et al. 2015. PLoS One. 10:117616.
Gene ID
13733 View all products for this Gene ID
UniProt
View information about F4/80 on UniProt.org

Related FAQs

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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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