- In1/CD74 (See other available formats)
- Regulatory Status
- Other Names
- CLIP, DHLAG, HLADG
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
|Cat #||Size||Price||Quantity Check Availability||Save|
CD74, also known as CLIP, is a 33.5kD homotrimer and functions as a MHC class II chaperone for exogenous peptides and other MHC class II molecules. CD74 binds MIF and regulates innate and adaptive immunity through CD44 signaling. It is found primarily on B-cells, monocytes, macrophages and dendritic cells, but can also be found on activated T-cells, endothelial and epithelial cells.Product Details
- Antibody Type
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
IHC-F, IP, WB - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This clone is also known as In1 and In-1. Additional reported applications (for the relevant formats) include: immunohistochemistry1, immunoprecipitation3, and western blotting4.
(PubMed link indicates BioLegend citation)
- Momburg F, et al. 1986. J. Immunol. 136:940. (IHC)
- Takahashi K, et al. 2009. Respir. Res. 10:33. (FC)
- Meza-Romero R, et al. 2014. J. Immunol. 192:4164. (IP)
- Heinrichs D, et al. 2011. Proc. Natl. Acad. Sci. USA 108:17444. (WB)
- Product Citations
AB_2566502 (BioLegend Cat. No. 151002)
- 33.5 kD and homotrimer.
B-cells, monocytes/macrophages, dendritic cells, activated T-cells, and activated endothelial/epithelial cells.
- MHC class II chaperone, MHC class II molecule sorting, and peptide loading.
- MHC class II molecules and peptides.
- Macrophage migration inhibitory factor (MIF).
- Cell Type
- B cells, Dendritic cells, Endothelial cells, Epithelial cells, Macrophages, Monocytes, T cells
- Biology Area
- Molecular Family
- CD Molecules, MHC Antigens
- Antigen References
1. Herrero L, et al. 2013. Arthritis Rheum. 65:2724.
2. Geneve L, et al. 2012. Int. Immunol. 24:645.
3. Sille FC, et al. 2011. Immunol Lett. 139:33.
4. Shih NY, et al. 1995. Mol. Immunol. 32:643.
5. Shi X, et al. 2006. Immunity 25:595.
6. Starlets D, et al. 2006. Blood 107:4807.
- Gene ID
- 16149 View all products for this Gene ID
- View information about CD74 on UniProt.org
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.