Purified anti-mouse CD74 (CLIP) Antibody

Pricing & Availability
Clone
In1/CD74 (See other available formats)
Regulatory Status
RUO
Other Names
CLIP, DHLAG, HLADG
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
In1_PURE_CD74_Antibody_FC_1_102715
C57BL/6 mouse splenocytes were stained with CD19 FITC and purified CD74 (clone In1/CD74) (top) or rat IgG2b, κ isotype control (bottom image), followed by anti-rat IgG2b Biotin and SAV-PE.
  • In1_PURE_CD74_Antibody_FC_1_102715
    C57BL/6 mouse splenocytes were stained with CD19 FITC and purified CD74 (clone In1/CD74) (top) or rat IgG2b, κ isotype control (bottom image), followed by anti-rat IgG2b Biotin and SAV-PE.
  • In1_PURE_CD74_Antibody_FC_2_102715
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151002 500 µg £188.00
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Description

CD74, also known as CLIP, is a 33.5kD homotrimer and functions as a MHC class II chaperone for exogenous peptides and other MHC class II molecules. CD74 binds MIF and regulates innate and adaptive immunity through CD44 signaling. It is found primarily on B-cells, monocytes, macrophages and dendritic cells, but can also be found on activated T-cells, endothelial and epithelial cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F, IP, WB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone is also known as In1 and In-1. Additional reported applications (for the relevant formats) include: immunohistochemistry1, immunoprecipitation3, and western blotting4.

Application References

(PubMed link indicates BioLegend citation)
  1. Momburg F, et al. 1986. J. Immunol. 136:940. (IHC)
  2. Takahashi K, et al. 2009. Respir. Res. 10:33. (FC)
  3. Meza-Romero R, et al. 2014. J. Immunol. 192:4164. (IP)
  4. Heinrichs D, et al. 2011. Proc. Natl. Acad. Sci. USA 108:17444. (WB)
Product Citations
  1. Baranov MV, et al. 2018. iScience. 11:160. PubMed
  2. Cho CS, et al. 2021. Cell. 184(13):3559-3572.e22. PubMed
RRID
AB_2566502 (BioLegend Cat. No. 151002)

Antigen Details

Structure
33.5 kD and homotrimer.
Distribution

B-cells, monocytes/macrophages, dendritic cells, activated T-cells, and activated endothelial/epithelial cells.

Function
MHC class II chaperone, MHC class II molecule sorting, and peptide loading.
Interaction
MHC class II molecules and peptides.
Ligand/Receptor
Macrophage migration inhibitory factor (MIF).
Cell Type
B cells, Dendritic cells, Endothelial cells, Epithelial cells, Macrophages, Monocytes, T cells
Biology Area
Immunology
Molecular Family
CD Molecules, MHC Antigens
Antigen References

1. Herrero L, et al. 2013. Arthritis Rheum. 65:2724.
2. Geneve L, et al. 2012. Int. Immunol. 24:645.
3. Sille FC, et al. 2011. Immunol Lett. 139:33.
4. Shih NY, et al. 1995. Mol. Immunol. 32:643.
5. Shi X, et al. 2006. Immunity 25:595.
6. Starlets D, et al. 2006. Blood 107:4807.

Gene ID
16149 View all products for this Gene ID
UniProt
View information about CD74 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06/03/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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